Protein Secretory Patterns during the Development of the Rat Ovary : Reproductive Biology
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概要
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Two dimensional polyacrylamide gel electrophoresis and autoradiography were used to study the time course of [35^S]methionine-labeled proteins accumulated in the incubation medium of rat ovaries during their development. In 18-days gestation rat fetuses, the ovary secreted polypeptide components very similar to those of the testis of male fetal rats, except that Sertoli cell secretory protein S70 was detected only in testicular samples. During postnatal development, polypeptides T35 (Mr 35,000/pI 4.67) and F37 (Mr 37,500/pI 4.63) continued to be expressed in the ovary throughout development. Antiserum against polypeptide S35, a component of Sertoli cell secretory S45-S35 heterodimeric protein, recognized immunoreactive sites in zona pellucida detected by indirect immunofluorescence. Results from this study demonstrate a contrast in the temporal expression of testicular and ovarian secretory polypeptides T35 and F37, and a possible antigenic homology between zona pellucida material and polypeptide S35.
- 社団法人日本動物学会の論文
- 1990-08-15
著者
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UEDA Hiroshi
Department of Pathology, School of Medicine Gifu University
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Fujimoto Sunao
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Fukui Yoshio
Department Of Molecular Oral Medicine And Maxillofacial Surgery Division Of Frontier Medical Science
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Ueda Hiroshi
Department Of Dermatology Fujita Health University School Of Medicine
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ARAKI HIDETOSHI
Department of Anatomy, University of Occupational and Environmental Health, School of Medicine
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Araki Hidetoshi
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Ueda Hiroshi
Department Of Anatomy University Of Occupational And Environmental Health School Of Medicine
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Ueda Hiroshi
Department Of Anatomy School Of Medicine University Of Occupational And Environmental Health
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