Genetically Engineered Active Qβ Replicase in Rabbit Reticulocyte Cell-Free System : a Fusion Protein of EF-Tu and EF-Ts Is Functional as the Subunit of Qβ Replicase
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概要
- 論文の詳細を見る
Qβ replicase functioning in Escherichia coli is an RNA-dependent RNA polymerase composed of one phage-coded subunit and three host-coded proteins : ribosomal protein S1,and protein elongation factors EF-Tu and EF-Ts. Qβ replicase lacking ribosomal protein S1 (α-less replicase) is capable of replicating some small RNAs. We attempted to create functional α-less replicase by co-expression of the mRNAs that code for the subunits of α-less replicase in a rabbit reticulocyte cell-free translation system. Replicase activity, however, could not be detected when both EF-Tu and EF-Ts were co-expressed with the phage-coded subunit. On the other hand, active α-less replicase was obtained when an EF-Ts-EF-Tu fusion protein was co-expressed with the phage-coded subunit. Consequently, we succeeded in generating genetically engineered active α-less Qβ replicase which functions in a eukaryotic cell-free system.
- 社団法人日本生物工学会の論文
- 2002-01-25
著者
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Nagamune Teruyuki
Department of Bioengineering, Graduate School of Engineering, The University of Tokyo
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Suzuki E
Shinshu Univ. Ueda Jpn
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WATANABE Kimitsuna
Department of Chemistry and Biotechnology,Graduate School of Engineering,The University of Tokyo
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Nagamune T
Univ. Tokyo Tokyo Jpn
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Nagamune T
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Nagamune Teruyuki
Dep. Of Chemistry And Biotechnology Graduate School Of Engineering The Univ. Of Tokyo 7-3-1 Hongo Bu
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Nagamune Teruyuki
Department Of Bioengineering Graduate School Of Engineering The University Of Tokyo:department Of Ch
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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SUZUKI Eiji
TDK Corporation
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FUKANO HAJIME
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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SUZUKI EIJI
Research Institute of Innovative Technology for the Earth
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Zako Tamotsu
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Nagamune Teruyuki
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo:dep
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Suzuki E
Tdk Corporation
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Fukano Hajime
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Watanabe Kimitsuna
Department Of Biological Sciences Faculty Of Bioscience And Biotechnology Tokyo Institute Of Technol
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Fukano Hazime
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo
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WATANABE Kimitsuna
Department of Agricultural Chemistry, Faculty of Agriculture, University of Tokyo
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