Novel Light-Illumination Scanning Tunneling Microscopy Equipped with Optical Fiber Probe
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概要
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Single molecular photocurrent generation was investigated using a novel light-illumination scanning tunneling microscope (LI-STM) equipped with an optical fiber probe. For this study, self-assembled monolayers (SAMs) of chimera proteins composed of cytochrome $b_{562}$ mutant proteins fused with an enhanced green fluorescent protein (EGFP) were prepared. With light illumination, the EGFP was excited, resulting in photocurrent generation due to the energy transfer to the cytochrome part instead of a typical fluorescence. Two different types of measurements were carried out: one employed a conventional LI-STM with a metallic probe and far-field optics, while the metallic probe was replaced with a laboratory-built doubly metal-coated optical fiber probe in another setup, where a local near-field illumination was possible. The comparison of these techniques indicated the superior sensitivity of the new technique to the conventional one, showing the evidence of the intramolecular energy transfer at a single molecular level.
- 2003-07-15
著者
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Nagamune Teruyuki
Department Of Bioengineering Center For Nanobio Integration The University Of Tokyo:department Of Ch
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Noh Jaegeun
Local Spatio-temporal Functions Laboratory Frontier Research System
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Lee Bumhwan
Department Of Chemistry And Biotechnology School Of Engineering The University Of Tokyo
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Takeda Shuji
Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo
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Hara Masahiko
Local Spatio-temporal Function Frontier Research System The Institute Of Physical And Chemical Resar
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Nakajima Ken
Local Spatio-temporal Functions Laboratory Frontier Research System Riken (the Institute Of Physical
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Noh Jaegeun
Local Spatio-Temporal Functions Laboratory, Frontier Research System, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
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Nagamune Teruyuki
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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Lee Bumhwan
Department of Chemistry and Biotechnology, Graduate School of Engineering, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-8656, Japan
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