Observation of Hybridization on a DNA Array by Surface Plasmon Resonace Imaging using Au Nanoparticles
スポンサーリンク
概要
- 論文の詳細を見る
To realize the sensitive detection of DNA hybridization, DNA-modified Au nanoparticles (DNA-AuNPs) containing single- and double-stranded portions were prepared. The hybridization of a target DNA using the DNA-AuNPs on a DNA self-assembled monolayer (DNA-SAM) was monitored in situ by surface plasmon resonance (SPR) imaging. A sandwich type hybridization (DNA-SAM/target DNA/DNA-AuNP) experiment was carried out in order to enhance the hybridization signal. SPR imaging results strongly indicate that the hybridization signal is enhanced several times compared to the case of target DNA hybridization.
- Published by the Japan Society of Applied Physics through the Institute of Pure and Applied Physicsの論文
- 2006-02-15
著者
-
Hara Masahiko
Local Spatio-temporal Function Frontier Research System The Institute Of Physical And Chemical Resar
-
Nakamura Fumio
Local Spatio-Temporal Functions Laboratory, RIKEN, 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
-
Manna Ablihito
Department of Materials Science, National University of Singapore, 10 Science Drive 4 Lower Kent Ridge Road, 117543, Singapore
-
Hara Masahiko
Local Spatio-Temporal Functions Lab., Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
-
Ito Masateru
Biophotonics Group, Photonic Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan
-
Tamada Kaoru
Biophotonics Group, Photonic Research Institute, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Ibaraki 305-8565, Japan
-
Knoll Wolfgang
Department of Materials Science, National University of Singapore, 10 Science Drive 4 Lower Kent Ridge Road, 117543, Singapore
-
Nakamura Fumio
Local Spatio-Temporal Functions Lab., Frontier Research System, RIKEN (The Institute of Physical and Chemical Research), 2-1 Hirosawa, Wako, Saitama 351-0198, Japan
関連論文
- Molecular Orientation in the Organized Molecular Films of Fluorinated Comb Polymers with Various Chain Lengths Studied by Soft X-ray Absorption Spectroscopy
- Different Adsorption States between Thiophene and α-Bithiophene Thin Films Prepared by Self-Assembly Method
- Bio-template Synthesis of Uniform CdSe Nanoparticles Using Cage-shaped Protein, Apoferritin
- Hybrid Scanning Near-Field Optical/Tunneling Microscopy with Indium-Tin-Oxide/Au Coated Optical Fiber Probe
- Solvent Effect on Formation of Cysteamine Self-Assembled Monolayers on Au(111)
- Characterization of COOH-Terminated Self-Assembled Monolayers and Adsorption Efficiency of DNA Molecules Studied by X-ray Photoelectron Spectroscopy and Near-Edge X-ray Absorption Fine Structure Spectroscopy
- Time-Dependent Phase Transition of Self-Assembled Monolayers Formed by Thioacetyl-Terminated Tolanes on Au(111)
- Outermost Surface Reactions of Molecular Thin Films Induced by Metastable-Atom Impacts
- Novel Light-Illumination Scanning Tunneling Microscopy Equipped with Optical Fiber Probe
- Single-Molecule Force Microscopy of Circularly Permuted Green Fluorescent Protein
- Observation of Hybridization on a DNA Array by Surface Plasmon Resonace Imaging using Au Nanoparticles
- Characterization of Organic/Metal Interfaces Using Angle-Resolved X-ray Photoelectron Spectroscopy