Kinetics of the interaction between DNA and acridine orange.
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概要
- 論文の詳細を見る
Both static and kinetic investigations have been performed on the interaction of calf thymus DNA with Acridine Orange (AO). The binding isotherm obtained spectrophotometrically was analyzed by a site exclusion theory and revealed the presence of two binding modes of dye molecule corresponding to intercalation and dimerization on DNA. In the kinetic experiments focusing on the intercalation mode by the temperature-jump technique, three relaxations were observed. From the analyses of both the relaxation time and amplitude, the two fast relaxations were ascribed to parallel binding reactions including a bimolecular pathway for direct transfer of dye molecule on the polymer and the slow relaxation was attributable to a conformational change of DNA molecules. The binding parameters kinetically obtained suggest that 80% of bound AO is in intercalated form and the remainder is in partially intercalated one as an external bound form.
- 公益社団法人 日本化学会の論文
著者
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Harada Shoji
Department Mechanical Engineering Kyushu Institute Of Technology
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Yasunaga Tatsuya
Department of Chemistry, Faculty of Science, Hiroshima University
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Sano Takayuki
Department of Chemistry, Faculty of Science, Hiroshima University
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Kure Naohisa
Department of Materials Science, Faculty of Science, Hiroshima University
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Harada Shoji
Department of Materials Science, Faculty of Science, Hiroshima University
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