Mechamical and Kinetic Properties of PVA Hydrogel Immobilizing β-Galactosidase
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概要
- 論文の詳細を見る
The mechanical kinetic properties of PVA (polyvinyl alcohol) hydrogel prepared by the interative freezing and thawing (IFT) method were studied in roder to assess its applicability as an immobilizing support. The PVA hydrogel showed rubber-like elasticity and the Young's modulus of the gel increased with increasing polymer concentration. The gel strength greatly improved with the number of freezing and thawing iterations(n) Thermal treatment of 10% gel with n=2 for 10 min at 45℃ caused a significant loss of strenth, but at n=7 no change in gel strength was observed. A β-galactosidase-producing recombinant E. coli was permeabilized by toluene and the kinetic characteristics of immobilized whole cells in the PVA hydrogel were investigated using ONPG (2-nitrophenyl-β-D-galactopyranoside) as a substrate under negligible intraparticle diffusion resistance. Compared with free cells, the Michaelis constant of the β-galactosidase was increased by the entrapment in PVA hydrogel, although that the free cells decreased with the addition of 3% PVA into the raction mixture. The kinetic parameters of both free and immobilized cells were not influenced by n until it reached 7 times, adn the PVA concentration did not affect the kinetic parameters of the immobilized cells. The optimal pH of the enzyme activity was not changed by immobilization, although the activity profile was broader than that of the free cells.
- 社団法人日本生物工学会の論文
- 1993-09-25
著者
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Nakazawa Yuko
Department of Cardiovascular and Respiratory Medicine, Shiga University of Medical Science
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Nakazawa Yuko
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Sano Tetsuya
Department of Pediatrics, Osaka University Graduate School of Medicine
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ARIGA OSAMU
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
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SANO YOSHIKI
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
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KATO Mitsuhiro
Department of Pediatrics, Yamagata University of School of Medicine
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Ariga O
Shinshu Univ. Nagano Jpn
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Ariga Osamu
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Sano Y
Shinshu Univ. Nagano Jpn
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Sano Y
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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SANO TAKAYUKI
Department of Pharmacology and Therapeutics, Faculty of Medicine, Oita University
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Sano Yoshiki
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Sano Tetsuya
Department Of Developmental Medicine (pediatrics) Osaka University Graduate School Of Medicine
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Sano Tetsuya
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Sano Takayuki
Department Of Pharmacology And Therapeutics Faculty Of Medicine Oita University
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Kato M
Morinaga Inst. Biological Sci. Yokohama Jpn
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Kato Mitsuhiro
Department Of Pediatrics Yamagata University Hospital
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Kato Mitsuhiro
Department Of Applied Chemistry Aichi Institute Of Technology
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ARIGA OSAMU
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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