Encapsulation of Biocatalyst with PVA Capsules

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概要

• 論文の詳細を見る

• PVA capsules were prepared by dropping PVA solution into cold methanol contained in a glass cylinder and subsequent incubation in methanol. The diameter of the capsules decreased slightly with incubation time due to dehydration. The membrane thickness of the capsules was measured by the immobilization of living cells and was found to increase almost proportionally to increasing incubation time in the methanol. Encapsulation of toluene-permeabilized cells having β-galactosidase activity as an enzyme was attempted under various conditions. The activity and stability of the enzyme in the capsules in phosphate buffer at 28℃ were examined. In a preliminary experiment, methanol inactivated the activity of the free enzyme. However, PVA repressed the inactivation. The activity of the capsules decreased with increasing incubation time, probably due to inactivation of the enzyme activity by methanol and the increase of intraparticle diffusion resistance as a result of growth in membrane thickness. Incubation of the capsules at 0℃ and a methanol temperature gradient in the cylinder used for the preparation of the capsules were effective in improving the enzyme activity of the capsules. The long-term stability of the enzyme activity of the capsules was better than that of free cells, and was improved by incubation at room temperature. Although a temperature gradient in the methanol used in capsule preparation enhanced the activity, it reduced the stability of the capsules.

• 社団法人日本生物工学会の論文
• 1994-07-25

著者

• Itoh Kohji

  Department of Medicinal Biotechnology, Institute of Health Biosciences, The University of Tokushima

• Itoh K

  Tokyo University Of Science

• ARIGA OSAMU

  Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit

• SANO YOSHIKI

  Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit

• Itoh K

  Faculty Of Industrial Science And Technology Tokyo University Of Science

• Itoh Kohji

  Department Of Biology Chiba University

• Ariga O

  Shinshu Univ. Nagano Jpn

• Ariga Osamu

  Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University

• Sano Y

  Shinshu Univ. Nagano Jpn

• Sano Y

  Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University

• Sano Yoshiki

  Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University

• NAGURA MASANOBU

  Department of Fine Materials Engineering, Faculty of Textile Science and Technology, Shinshu Univers

• Itoh Kohji

  Department Of Applied Electronics Science University Of Tokyo

• ARIGA OSAMU

  Department of Chemical Engineering, Faculty of Engineering, Nagoya University

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