Immobilization of a Thermostable Enzyme Using a Sol-Gel Preparation Method
スポンサーリンク
概要
- 論文の詳細を見る
A thermostable β-galactosidase was immobilized with tetramethoxysilane (TMOS) using a sol-gel preparation method. The thermal stability of free and immobilized enzymes changed with enzyme concentration, suggesting an effect of contaminants in the enzyme solution. The thermal stability of the immobilized enzyme was affected by preparation conditions and was much lower than that of free enzyme. The use of high concentrations of magnesium in the preparation enhanced the thermal stability of the immobilized enzyme, although high concentrations of magnesium in the incubation buffer reduced the thermal stability. A cell-free extract of E. coli B cells exhibiting no β-galactosidase activity was found to be most effective in improving the thermal stability of both the free and immobilized enzyme. The pH of incubation buffer during thermal treatment and preparation of immobilized enzyme significantly affected enzyme activity and thermal stability. The highest activity and stability were obtained at pH 6. Immobilization and thermal treatment had no effect on the Michaelis constants independent of the enzyme concentration and this suggests that immobilizing support and the cell-free extracts have insignificant effect on the kinetic properties of the immobilized enzyme.
- 1996-10-25
著者
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SUZUKI Tomoharu
Department of Astronomy, University of Tokyo
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MURAKAMI Yasushi
Department of Fine Materials Engineering, Faculty of Textile Science and Technology, Shinshu Univers
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Suzuki Tomoharu
Department Of Astronomy University Of Tokyo
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Suzuki Tomoharu
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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ARIGA OSAMU
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
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SANO YOSHIKI
Department of Fine Material Engineering, Faculty of Texile Science and Technology, Shinshu Universit
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Ariga Osamu
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Ariga Osamu
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Sano Yoshiki
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Murakami Yasushi
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Sano Yoshiki
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Murakami Yasushi
Department Of Fine Material Engineering Faculty Of Textile Scinece And Technology Shinshu University
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Murakami Y
Ntt Access Network Serv. Systems Lab. Tsukuba‐shi Jpn
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ARIGA OSAMU
Department of Chemical Engineering, Faculty of Engineering, Nagoya University
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