Effect of immobilization on stability of recombinant plasmids in gene-engineered microorganisms.
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概要
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Stability of a recombinant plasmid pMCT98 was investigated for free and immobilized Escherichia coli C600/pMCT98 cells. KC1, a hardening reagent for immobilizing matrix, inhibited cell growth and decreased the stability of the plasmid for free cells. However, the immobilization within ic-carrageenan gel did not affect cell growth and increased significantly the stability of the plasmid, and the amount of the gene product did not decrease through successive passage of the immobilized cells. Coexistence of plasmid pRLK13 lowered the stability of plasmid pMCT98 for both free and immobilized cells. Some oscillations in the ratio of cells harboring the plasmids were observed in the immobilized E. coli C600/pMCT98/pRLK13 system.
- 公益社団法人 化学工学会の論文
著者
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Ariga Osamu
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Kobayashi Takeshi
Department Of Biological Chemistry College Of Bioscience And Biotechnology Chubu University
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Sano Yoshiki
Department Of Fine Material Engineering Faculty Of Texile Science And Technology Shinshu University
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Takagi Hiromi
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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Ando Masataka
Department Of Astronomy Kyoto University
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Watari Tohru
Department Of Fine Materials Engineering Faculty Of Textile Science And Technology Shinshu Universit
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IIJIMA Shinji
Department of Agricultural Chemistry, The University of Tokyo
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ANDO MASATAKA
Department of Fine Materials Engineering, Shinshu University
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TAKEUCHI KIMIHISA
Department of Fine Materials Engineering, Shinshu University
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SANO YOSHIKI
Department of Fine Materials Engineering, Shinshu University
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