Isolation and Sequencing of a cDNA Clone Encoding a 20-kDa Protein with Trypsin Inhibitory Activity
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概要
- 論文の詳細を見る
The amino acid sequence of a novel trypsin inhibitor (p20) was completed by the molecular cloning of the protein in cultured soybean cells. The clone nucleotide contains an open reading frame encoding a polypeptide of 206 amino acids that shows 45-50% sequence homology to members of the Kunitz-type trypsin inhibitor family. The p20 transcript is expressed in the roots, stems and leaves of soybean seedlings. DNA gel blot analyses show that the p20 in soybean is encoded by a single gene, and that this gene may not contain an intron.
- 社団法人日本農芸化学会の論文
- 2000-06-23
著者
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Hirota Tomoko
Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University
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HIRATA Toshifumi
Department of Chemistry, Faculty of Science, Hiroshima University
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ASHIDA Yoshiyuki
Department of Biochemistry, School of Dentistry, Tokushima University
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Ashida Yoshiyuki
Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University
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Ashida Yoshiyuki
Department Of Biochemistry School Of Dentistry Tokushima University
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Hirata T
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Hirata T
Hiroshima Univ. Higashi‐hiroshima Jpn
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Hirata Toshifumi
Dep. Of Mathematical And Life Sciences Graduate School Of Sci. Hiroshima Univ.
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MATSUSHIMA Akihito
Department of Mathematical and Life Science, Graduate School of Sciense, Hiroshima University
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TSURU Yukari
Department of Mathematical and Life Sciences, Graduate School of Science, Hiroshima University
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Matsushima Akihito
Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University
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Tsuru Yukari
Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University
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Hirata Toshifumi
Department Of Chemistry Faculty Of Science Hiroshima University
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