Characterization of Recombinant p20 Trypsin Inhibitor, a New Protein from Glycine max

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概要

• 論文の詳細を見る

• A 20-kDa protein (p20) having 40% sequence similarity with Kunitz-type soybean trypsin inhibitor (STI) from Glycine max (soybean) cultured cells was expressed in Escherichia coli. The recombinant p20 (rp20) inhibited the activity of trypsin at the same level as STI (rp20, Ki=50nM; STI, Ki=75nM), and both rp20 and STI displayed non-competitive inhibition of trypsin activity. Although STI inhibited the activity of α-chymotrypsin (Ki=140nM) and elastase (Ki=207nM), rp20 did not inhibit the activity of α-chymotrypsin and elastase. These results show that p20 is a novel type of trypsin inhibitor.

• 日本植物細胞分子生物学会の論文
• 2003-03-03

著者

• Hirota Tomoko

  Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University

• HIRATA Toshifumi

  Department of Chemistry, Faculty of Science, Hiroshima University

• WATANABE Junko

  Department of Public Health, Yokohama City University School of Medicine

• ASHIDA Yoshiyuki

  Department of Biochemistry, School of Dentistry, Tokushima University

• Ashida Yoshiyuki

  Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University

• Ashida Yoshiyuki

  Department Of Biochemistry School Of Dentistry Tokushima University

• Hirata T

  Department Of Mathematical And Life Sciences Graduate School Of Science Hiroshima University

• Hirata T

  Hiroshima Univ. Higashi‐hiroshima Jpn

• Hirata Toshifumi

  Dep. Of Mathematical And Life Sciences Graduate School Of Sci. Hiroshima Univ.

• Watanabe J

  Food Function Division National Food Research Institute

• MATSUSHIMA Akihito

  Department of Mathematical and Life Science, Graduate School of Sciense, Hiroshima University

• Watanabe J

  Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University

• Matsushima Akihito

  Department Of Mathematical And Life Science Graduate School Of Sciense Hiroshima University

• Watanabe Junko

  Department Of Ophthalmology Surugadai Nihon University Hospital

• Watanabe Junko

  Department Of General Education Kinki University

• Hirata Toshifumi

  Department Of Chemistry Faculty Of Science Hiroshima University

• WATANABE JUNKO

  Department of Biochemistry, Saitama Cancer Center Research Institute

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