Water Permeability of Plasma Membranes of Cultured Rice, Grape, and CH27 Cells Measured Dielectrically

概要

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The capacitance of suspensions of cultured rice cells (Oryza sativa L. ssp. japonica), grape cells (Vitis sp.), and CH27 cells originated from murine B-cell lymphoma was measured in the frequency range of 0.2 to 10 MHz. The relationship between the increase in capacitance caused by the presence of cells at 0.4 MHz, ΔC, and the cell density was linear. Measurement of capacitance was useful in measurement of transitional changes in cell volume under external osmotic stress when sucrose was added. From the course of volume changes with such stress, the water permeabilities of the plasma membrane, L_p, were measured to be 0.015, 0.020, and 0.090 pm/(s・Pa) at 25℃, for rice cells, grape cells, and CH27 cells, respectively. The smaller L_p for plant cells seemed to explain why preservation of plant cells by freezing is more difficult than for animal cells. From the temperature dependence of L_p, the apparent activation energies were calculated to be 12.0±2.9 and 13.0±5.2 kcal/mol for rice cells and CH27 cells, respectively.
社団法人日本農芸化学会の論文
1997-11-23