Fractal Analysis of Aggregates Formed by Heating Dilute BSA Solutions Using Light Scattering Methods
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概要
- 論文の詳細を見る
The structure of aggregates formed by heating dilute BSA solution was analyzed with the fractal concept using light scattering methods. BSA was dissolved in HEPES buffer of pH 7.0 and acetate buffer of pH 5.1 to 0.1% and 0.001% solutions, respectively, and heated at 95℃, varying the heating time t_a. The fractal dimension D_f of the aggregate in the solution was evaluated from static light scattering experiments. The polydispersity exponent τ and the average hydrodynamic radius <R_h> of the aggregates were calculated from dynamic light scattering experiments using master curves obtained by Klein et al. The values of D_f and τ of heat-induced aggregates of BSA at pH 7.0 were about 2.1 and 1.5, respectively, the values of which agreed with those predicted by the reaction-limited cluster-cluster aggregation (RLCCA) model. On the other hand, D_f of heat-induced aggregates at pH 5.1 was about 1.8, which agreed with that predicted by the diffusion-limited cluster-cluster aggregation (DLCCA) model. The dependence of <R_h> for the sample of pH 7.0 on t_a was similar to that of the polystyrene colloids reported previously.
- 社団法人日本農芸化学会の論文
- 1996-11-23
著者
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KUMAGAI Hitoshi
Department of Food Science and Nutrition, Kyoritsu Women’s University
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Hagiwara Tomoyuki
Nutritional Science Laboratory Morinaga Milk Industry Co. Ltd.
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Hagiwara Tomoyuki
森永乳業
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Hagiwara Tomoyuki
Nutritional Science Laboratory Morinaga Milk Industry Co.
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NAKAMURA KOZO
Department of Orthopaedic Surgery, Faculty of Medicine, The University of Tokyo
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中村 薫
京大 化研
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Nagahama Kazuhiro
Department Of Applied Microbial Technology The Kumanoto Institute Of Technology
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Kumagai Hitoshi
Central Research Institute Meiji Milk Products Co. Ltd.:(present Office)department Of Agricultural C
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Kumagai Hitoshi
Department Of Agricultural Chemistry The University Of Tokyo
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Nakamura Kozo
Department Of Bioscience And Biotechnology Faculty Of Agriculture Shinshu University
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HAGIWARA Tomoaki
Department of Food Science and Technology, Tokyo University of Fisheries
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Kurihara H
Meiji Seika Kaisha Ltd. Yokohama Jpn
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Nakamura Kahoru
Department Of Nutrition Tokyo University Of Agriculture
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Kumagai Hitoshi
Department Of Agricultural And Biological Chemistry Nihon University
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Hagiwara Tomoaki
Department Of Food Science And Technology Tokyo University Of Marine Science And Technology
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Nakamura Kozo
Department Of Agricultural Chemistry The University Of Tokyo
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熊谷 仁
共立女子大学家政学部
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Hagiwara Tomoaki
Department of Applied Biological Chemistry, Division of Agriculture and Agricultural Life Sciences, The University of Tokyo:(Present address)Department of Food Science and Technology, tokyo University of Fisheries
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