Production of Protein Kinase C-δ by the Baculovirus-Insect Cell System in Serum-Supplemented and Serum-Free Media
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概要
- 論文の詳細を見る
When rat protein kinase C-δ (PKC-δ) was produced by Sf9 cells infected with a recombinant baculovirus in shake-flask culture using a serum-containing medium, the intracellular PKC-δ content decreased in the late period while the extracellular PKC-δ markedly increased. During the late period of serum-free culture, the extracellular PKC-δ level considerably declined, but the addition of a protease inhibitor, leupeptin, prevented the reduction in PKC-δ production.
- 社団法人日本生物工学会の論文
- 2003-02-25
著者
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山地 秀樹
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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山地 秀樹
神戸大・工
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Yamaji Hideki
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University
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FUKUDA HIDEKI
Division of Molecular Science, Graduate School of Science and Technology, Kobe University
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HIRAKAWA DAIGO
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University
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TAGAI SHIN-ICHI
Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University
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Fukuda H
Division Of Molecular Science Graduate School Of Science And Technology Kobe University
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Fukuda Hideki
Organization Of Advanced Science And Technology Kobe University
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Fukuda H
Kobe Univ. Kobe
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Fukuda Hideki
Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K
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Fukuda H
Kobe University
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Yamaji H
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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Hirakawa Daigo
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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Fukuda H
Kobe Univ. Kobe Jpn
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Fukuda Hideki
Division Of Molecular Science Graduate School Of Science And Technology Kobe University
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Tagai Shin-ichi
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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Yamaji Hideki
Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University
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Fukuda Hideo
Dept. Appl. Microbial Technology Kumamoto Institute Of Technology
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Fukuda Hideki
Division Of Bioscience The Graduate School Of Science And Technology Kobe University
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