Affinity Purification and Immobilization of Fusion Chaperonin GroEL-(His)_6 and Its Utilization to Mediate Protein Refolding

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概要

• 論文の詳細を見る

• Escherichia coli chaperonin GroEL with a hexa-histidine affinity tag(His)_6 fused to its C-terminal(GroEL-(His)_6)was overproduced in E.coli. GroEL-(His)_6 was expressed in a soluble form in E.coli and purified to homogeneity in a single step by nickel chelate resin. GroEL-(His)_6 was found to form a tetradecamer and showed a similar ATPase activity to native GroEL. GroEL-(His)_6 mediated refolding of guanidine hydrochloride-unfolded yeast enolase and reactivation of thermally inactivated yeast enolase to similar extents as native GroEL. The structure and function of GroEL are thus little affected by the fusion of the(His)_6 tag to the C-terminal of GroEL. GroEL-(His)_6 was efficiently immobilized on nickel chelate Cellulofine, and immobilized GroEL-(His)_6 retained a high ability to mediate protein refolding. Therefore, GroEL-(His)_6 is advantageous in the application of chaperonin to protein refolding systems.

• 社団法人日本生物工学会の論文
• 1998-10-25

著者

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• Fukuda Hideki

  Organization Of Advanced Science And Technology Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Fukuda H

  Kobe Univ. Kobe Jpn

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• TESHIMA TADANARU

  Division of Bioscience, Kobe University

• MASHIMO SHINOBU

  Division of Molecular Science, Kobe University

• KONDO AKIHIKO

  The Graduate School of Science and Technology, and Department of Chemical Science and Engineering, F

• Mashimo Shinobu

  Division Of Molecular Science Kobe University

• Teshima Tadanaru

  Division Of Bioscience Kobe University

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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