Use of a Substrate as an Encapsulated Marker for Liposome Immunoassay

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概要

• 論文の詳細を見る

• For spectrophotometric liposome immunoassay of a specific antibody, antigen (cytochrome c)-coupled liposomes containing the substrate glucose-6-phosphate (G6P) as a marker were prepared from 1,2-di(3RS, 7R, 11R-phytanyl)-sn-glycero-3-phosphocholine (DPhyPC). DPhyPC liposomes showed good stability against G6P leakage, which was below 8% after incubation for 70 d at 4℃. When cytochrome c-coupled liposomes were incubated with anti-cytochrome c antibody and complement, after which glucose-6-phosphate dehydrogenase and NAD^+ were added, the absorbance at 340 nm increased with the amount of anti-cytochrome c antibody added.

• 社団法人日本生物工学会の論文
• 1997-06-25

著者

• Kondo Akihiko

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• Nakagawa Masato

  Department Of Hospital Pharmacy Faculty Of Pharmaceutical Sciences Kyoto Pharmaceutical University

• 山地 秀樹

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Yamaji Hideki

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Yamaji H

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Nakagawa Masato

  Graduate School Of Science And Technology Kobe University

• TOMIOKA KANJI

  Process Technology and Research Laboratories, Research Institute, Kaneka Corporation

• Fukuda H

  Kobe Univ. Kobe Jpn

• Tomioka Kanji

  Process Technology And Research Laboratories Research Institute Kaneka Corporation

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Kondo Akihiko

  Department Of Bioactive Molecules National Institute Of Infectious Diseases

• Yamaji Hideki

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Nakagawa Masato

  Department Of Botany Graduate School Of Science Kyoto University

• Nakagawa M

  Graduate School Of Science And Technology Kobe University

• Kondo Akihiko

  Department Of Applied Chemistry Kyushu Institute Of Technology

• Kondo Akihiko

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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