Effect of Oxidized and Reduced Forms of Escherichia coli DsbC on Protein Refolding

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概要

• 論文の詳細を見る

• DsbC, which catalyzes disulfide isomerization, was overproduced in the periplasm of Escherichia coli and purified from the periplasmic fraction by osmotic shock and anion-exchange chromatography. The active site of the purified DsbC was found to be an oxidized form (ox-DsbC) which could be converted to the reduced form (red-DsbC) by the addition of dithiothreitol. The effect of ox- and red-DsbC on the refolding of chemically denatured and reduced proteins with different numbers of disulfide bonds and free cysteine-thiol groups was investigated. Ox-DsbC facilitated the refolding of proteins with multiple disulfide bonds in both oxidative and reductive environments, while red-DsbC facilitated refolding only in the former. On the other hand, only red-DsbC facilitated the refolding of proteins with multiple free cysteine-thiol groups but either form of DsbC did not facilitate the refolding of proteins with only one cysteine-thiol group. It is therefore important to choose the form which suits the properties of the protein. Holo-chaperonin from Thermus thermophilus and DsbC demonstrated a synergistic effect on protein refolding.

• 社団法人日本生物工学会の論文
• 2002-08-25

著者

• Kondo Akihiko

  Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• Fukuda H

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Fukuda Hideki

  Organization Of Advanced Science And Technology Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Fukuda H

  Kobe University

• Fukuda H

  Kobe Univ. Kobe Jpn

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• KOHDA JIRO

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• KAWAHARA NOBORU

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• 近藤 昭彦

  神戸大 大学院工学研究科

• Kondo Akihiko

  Department Of Bioactive Molecules National Institute Of Infectious Diseases

• Kohda J

  Hiroshima City University

• Kohda Jiro

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University:(present Add

• Kawahara Noboru

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Kondo Akihiko

  Department Of Applied Chemistry Kyushu Institute Of Technology

• Kondo Akihiko

  Department Of Applied Chemistry Faculty Of Engineering Kyushu Institute Of Technology

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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