Effects of Phospholipids on Growth of Chinese Hamster Ovary Cells in Serum-Free Media

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概要

• 論文の詳細を見る

• The effects of phosphatidic acid (PA) and lysophosphatidic acid (LPA) dispersed in serum-free media on the growth of Chinese hamster ovary (CHO) cells were investigated. After cells were incubated in serum-free media supplemented with PA or LPA for 3 d, the cellular growth was evaluated by the MTT (3-(4, 5-dimethylthiazol1-2-yl)-2, 5-diphenyltetrazolium bromide) assay. Supplementing PA from egg yolk lecithin to basal synthetic media such as α-MEM (minimum essential medium, alpha modification) markedly promoted the growth of CHO cells. PA from egg yolk lecithin also enhanced the cell growth in a serum-free medium containing insulin and transferrin. When PA analogues with defferent acyl chains of C14-18 were compared, PA with unsaturated acyl chains, dioleoly-PA (C18:1), was most effective for stimulating the growth of CHO cells. Similar results were obtained when LPA was examined. These results suggest that PA and LPA, especially those with unsaturated acyl chains, are promising growth-promoting supplements for use as costituents in a low-protein serum-free medium.

• 社団法人日本生物工学会の論文
• 1999-09-25

著者

• 山地 秀樹

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Yamaji Hideki

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• SAKAI KENTARO

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• FUKUDA HIDEKI

  Division of Molecular Science, Graduate School of Science and Technology, Kobe University

• MATSUNAGA TOSHIO

  Department of Chemical Science and Engineering, Faculty of Engineering, Kobe University

• Sakai Kentaro

  岡山県立大学 保健福祉学部栄養学科

• Sakai Kentaro

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Fukuda Hideki

  Organization Of Advanced Science And Technology Kobe University

• Fukuda H

  Kobe Univ. Kobe

• Fukuda Hideki

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Yamaji H

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda H

  Kobe Univ. Kobe Jpn

• Fukuda Hideki

  Division Of Molecular Science Graduate School Of Science And Technology Kobe University

• Sakai K

  Department Of Molecular Science And Material Engineering Graduate School Of Science And Technology K

• Matsunaga Toshio

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Yamaji Hideki

  Department Of Chemical Science And Engineering Faculty Of Engineering Kobe University

• Fukuda Hideo

  Dept. Appl. Microbial Technology Kumamoto Institute Of Technology

• Sakai Kentaro

  Dep. Of Nutrition And Health Promotion Fac. Of Human Life Sci. Hiroshima Jogakuin Univ.

• Sakai Kensuke

  Department Of Nutrition And Health Promotion Faculty Of Human Life Science Hiroshima Jogakuin Univer

• Fukuda Hideki

  Division Of Bioscience The Graduate School Of Science And Technology Kobe University

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