The Specific Expression Patterns of Lactase, Sucrase and Calbindin-D9k in Weaning Rats Are Regulated at the Transcriptional Level
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概要
- 論文の詳細を見る
During weaning, rat lactase-phlorizin hydrolase (LPH) expression decreased to the low levels found in adults, while sucrase-isomaltase (SI) sharply increased. Calbindin-D9k (CaBP) is specific to the intestine and expression peaked within a few days of weaning. The present study investigates whether these molecules are regulated at transcriptional or post-transcriptional levels and examines the effects of diet on regulation. At normal weaning on day 21, litters were separated from their dams and one group was fed with a standard laboratory diet (weaned (W) group). The other group received a diet containing lactose as the sole source of carbohydrate (lactose-fed (L) group). Mucosal cells were obtained from the proximal part of the rat small intestine and then the activity and concentration of LPH, SI and CaBP proteins and mRNAs were determined. Three parameters revealed the same changing patterns in LPH, SI and CaBP during development and there was significant (p<0.001) correlation between three parameters : LPH, r=0.97 for activity vs. protein, r=0.99 for activity vs. mRNA, r=0.96 for protein vs. mRNA, SI, r=0.99 for activity vs. protein, r=0.98 for activity vs. mRNA, r=0.96 for protein vs. mRNA, CaBP, r=0.94 for activity vs. protein, r=0.97 for activity vs. mRNA, r=0.95 for protein vs. mRNA. Expression of the three proteins did not differ between the L and W groups. Accordingly, it has been suggested that the expression of LPH, SI and CaBP during development is defined at the transcriptional level and dietary changes do not exert a primary effect on it.
- 日本ビタミン学会の論文
著者
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GODA Toshinao
Department of Nutrition, School and Nutritional Sciences, The University of Shizuoka
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Fukushima Akiko
Department of Neuroscience and Neurology, Osaka City University Medical School
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Miwa Yoshikazu
Dep. Of Clinical Pharmacology Fac. Of Medical Sciences Kyushu Univ. Jpn
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Miwa Yoshikazu
Department Of Clinical Pharmacology Graduate School Of Medical Sciences Kyushu University
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Motohashi Y
Department Of Internal Medicine Keio University School Of Medicine
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Motohashi Yoshiko
慶応義塾大学 医学部
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Sakai Kentaro
岡山県立大学 保健福祉学部栄養学科
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Goda T
Department Of Nutrition School Of Food And Nutritional Sciences The University Of Shizuoka
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Goda Toshinao
Laboratories Of Nutritional Physiology School Of Food And Nutritional Sciences The University Of Shi
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Goda Toshinao
Department Of Nutrition School And Nutritional Sciences The University Of Shizuoka
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SAKUMA Keiko
Department of Molecular Nutrition, Kagawa Nutrition University
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Fukushima Akiko
Department Of Molecular Nutrition Kagawa Nutrition University
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Fukushima Akiko
Department Of Director Nursing Fukuda Hospital
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Fukushima Akiko
National Institute Of Advanced Industrial Science And Technology (aist) Spintronics Research Center
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Sakai Kentaro
Dep. Of Nutrition And Health Promotion Fac. Of Human Life Sci. Hiroshima Jogakuin Univ.
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MOTOHASHI Yasuko
Department of Molecular Nutrition, Kagawa Nutrition University
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Sakai Kensuke
Department Of Nutrition And Health Promotion Faculty Of Human Life Science Hiroshima Jogakuin Univer
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Goda Toshinao
Department Of Food And Nutritional Sciences And Global Center Of Excellence (coe) Program University
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Sakuma Keiko
Department Of Internal Medicine Tokai University School Of Medicine
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Motohashi Yasuko
Department Of Molecular Nutrition Kagawa Nutrition University
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FUKUSHIMA Akiko
Department of Biochemistry, Saitama Medical School, Moroyama, Iruma-gun
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