Expression of Calbindin-D9k, VDR and Cdx-2 Messenger RNA in the Process by Which Fructooligosaccharides Increase Calcium Absorption in Rats

スポンサーリンク

概要

• 論文の詳細を見る

• We have previously shown, through evidence that the expression of calbindin-D9k protein shows a dose-dependent change in the intestine, that calbindin-D9k plays a role in the ability of a fructooligosaccharide (FOS) diet to increase calcium absorption. This study shows that the regulation of calbindin-D9k expression occurs at the transcriptional level in a segment-specific manner, decreasing in the proximal intestine and increasing in the colorectal segment. To determine the transcriptional regulation of the FOS diet on calbindin-D9k expression, two transcription factors, vitamin D receptor (VDR) and cdx-2, were analyzed during 10 d feeding of the FOS diet. The mRNA expression of VDR and cdx-2 was influenced by the FOS diet and showed a segment-specific change. In the proximal small intestine, there was a significant correlation between the changes in both mRNAs (r=0.69, p<0.01), while the expression of calbindin-D9k correlated neither with VDR nor with cdx-2. This means that the transcriptional change induced by the FOS diet was not regulated by VDR and cdx-2. In the colorectal segment, there were significant correlations between gene expressions of calbindin-D9k vs. VDR, r=0.73, p<0.01 and calbindin-D9k vs. cdx-2, r=0.52, p<0.05. These results suggested that both transcription factors, VDR and cdx-2, were involved in the regulation of calbindin-D9k gene expression in the colorectal segment during the process through which the FOS diet enhanced calcium absorption.

• 日本ビタミン学会の論文

著者

• Fukushima Akiko

  Department of Neuroscience and Neurology, Osaka City University Medical School

• Ohta Atsutane

  香川栄養大学

• Ohta Atsutane

  Department Of Applied Nutrition Josai International University

• Ohta Atsutane

  Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.

• Sakai Kentaro

  岡山県立大学 保健福祉学部栄養学科

• SAKAI Kensuke

  Department of Applied Nutrition, Josai International University

• SAKUMA Keiko

  Department of Molecular Nutrition, Kagawa Nutrition University

• Fukushima Akiko

  Department Of Molecular Nutrition Kagawa Nutrition University

• Fukushima Akiko

  Department Of Director Nursing Fukuda Hospital

• Fukushima Akiko

  National Institute Of Advanced Industrial Science And Technology (aist) Spintronics Research Center

• Sakai Kentaro

  Dep. Of Nutrition And Health Promotion Fac. Of Human Life Sci. Hiroshima Jogakuin Univ.

• Sakai Kensuke

  Department Of Nutrition And Health Promotion Faculty Of Human Life Science Hiroshima Jogakuin Univer

• Sakuma Keiko

  Department Of Internal Medicine Tokai University School Of Medicine

• FUKUSHIMA Akiko

  Department of Biochemistry, Saitama Medical School, Moroyama, Iruma-gun

関連論文

• ＜Symposium IV＞Oligomeric Aβ is the sole culprit molecule to cause Alzheimer's disease?
• Cell Cycle Analysis of Chinese Hamster Ovary Cells Stimulated by Phosphatidic Acid in Serum-Free Culture(CELL AND TISSUE ENGINEERING)
• Comparative Study on Delivery of Phosphatidic Acid to Serum-Free Culture of Chinese Hamster Ovary Cells(ANIMAL BIOTECHNOLOGY)
• Use of Nonionic Surfactants for Effective Supply of Phosphatidic Acid in Serum-Free Culture of Chinese Hamster Ovary Cells
• Production of Recombinant Protein by Baculovirus-Infected Insect Cells in Immobilized Culture Using Porous Biomass Support Particles
• Effects of Phospholipids on Growth of Chinese Hamster Ovary Cells in Serum-Free Media
• Evidence That the Expression of the Gene for NADPH-Cytochrome P-450 Reductase is n-Alkane-inducible in Candida maltosa
• Evolutionary Position of n-Alkane-assimilating Yeast Candida maltosa Shown by Nucleotide Sequence of Small-subunit Ribosomal RNA Gene
• Phosphatidylserine Synthesis Required for the Maximal Tryptophan Transport Activity in Saccharomyces cerevisiae
• Isolation and Characterization of ECT1 Gene Gncoding CTP: Phosphoethanolamine Cytidylyltransferase of Saccharomyces cerevisiae^1
• Expression of Calbindin-D9k, VDR and Cdx-2 Messenger RNA in the Process by Which Fructooligosaccharides Increase Calcium Absorption in Rats
• The Effect of Fructooligosaccharides was Analyzed by cDNA Expression Arrays
• Electronic Structure of Ce_Nd_xO_ Probed by Soft-X-Ray Spectroscopy
• Band Structure of Sr_Ba_Nb_2O_6 Thin Film Probed by Soft-X-Ray Emission Spectroscopy
• Electronic Structure of Delafossite-Type Oxide PtCoO_2 by Resonant-Photoemission Spectroscopy
• Band Structure of TiO_2-Doped Yttria-Stabilized Zirconia Probed by Soft-X-Ray Spectroscopy
• Cloning and Characterization of a Chitinase-encoding Gene (chiA) from Aspergillus nidulans, Disruption of Which Decreases Germination Frequency and Hyphal Growth
• Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
• Isolation and Characterization of Two Chitin Synthase Genes from Aspergillus nidulans
• Effect of Citrus Fruit (Sudachi) Juice on Absorption of Calcium from Whole Small Fish in Healthy Young Men
• Japanese Citrus Fruit (Sudachi) Juice Is Associated with Increased Bioavailability of Calcium from Whole Small Fish and Suppressed Bone Resorption in Rats
• IgE Responses in Mice Fed Moderate Protein Deficient and High Protein Diets
• Low-Protein Diet Induces Oral Tolerance to Ovalbumin in Mice
• Effects of pH Variation and NaCl on In Vitro Digestibility of Cow's Milk Proteins in Commercially Available Infant Formulas
• Construction and Some Characterization of a Yeast Artificial Chromosome Library from DNA of a Tomato Line Having Four Disease Resistance Traits
• Identification of cDNA Clones Coding for the Style Specific S_-Glycoprotein Gene Associated with Gametophytic Self-incompatibility in Tomato (Lycopersicon peruvianum)
• Partial Sequence of Acid Phosphatase-1^1 Gene (Aps-1^1) Linked to Nematode Resistance Gene (Mi) of Tomato
• Pseudohyphal Growth in a Dimorphic Yeast, Candida maltosa, after Disruption of the C-GCN4 Gene, a Homolog of Saccharomyces cerevisiae GCN4(Biochemistry & Molecular Biology)
• Employment of the Human Estrogen Receptor β Ligand-Binding Domain and Co-Activator SRC1 Nuclear Receptor-Binding Domain for the Construction of a Yeast Two-Hybrid Detection System for Endocrine Disrupters
• Evidence That the Aspergillus nidulans Class I and Class II Chitin Synthase Genes, chsC and chsA, Share Critical Roles in Hyphal Wall Integrity and Conidiophore Development^1
• Cloning and Characterization of EPD2,a Gene Required for Efficient Pseudohyphal Formation of a Dimorphic Yeast, Candida maltosa
• Isolation of a Chitin Synthase Gene (chsC) of Aspergillus nidulans
• Isolation and Characterization of Two Chitin Synthase Genes of Rhizopus oligosporus
• Low Dose Iron Supplementation with Fructooligosaccharides Improves Iron Status and Aerobic Capacity in Women under Moderate Physical Training
• Effect of Dietary Short-Chain Fructooligosaccharides on the Cecal Microflora in Gastrectomized Rats
• Fluorescent In Situ Hybridization Analysis of Cecal Microflora in Rats Simultaneously Administrated Lactobacillus rhamnosus KY-3 and Cellobiose
• Effect of Lactobacillus rhamnosus KY-3 and Cellobiose as Synbiotics on Lipid Metabolism in Rats
• The Specific Expression Patterns of Lactase, Sucrase and Calbindin-D9k in Weaning Rats Are Regulated at the Transcriptional Level
• Transcriptional Regulation of Rat Calbindin Expression during Development Determined by Bacterially Expressed Protein
• Thrombospondin-1 promotes fibroblast-mediated collagen gel contraction caused by activation of latent transforming growth factor β-1
• Effects of Lower Limb and Dorsolumbar Massages on Edema in Postpartum Women
• Yeast Two-Hybrid Detection Systems That Are Highly Sensitive to a Certain Kind of Endocrine Disruptors
• The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
• Gene Regulation in Response to Overexpression of Cytochrome P450 and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
• Misoflded Membrane-Bound Cytochrome P450 Activates KAR2 Induction through Two Distinct Mechanisms
• Null Mutation in IRE1 Gene Inhibits Overproduction of Microsomal Cytochrome P450Alk1 (CYP 52A3) and Proliferation of the Endoplasmic Reticulum in Saccharomyces cerevisiae
• Regulation of Phospholipid Synthesis in Response to Nutritional Environments in Mitochondria of Saccharomyces cerevisiae
• Effect of amino acid mixtures on nasal allergic responses induced by toluene diisocyanate in mice
• Inflammatory myofibroblastic tumor of the uterus with prominent myxoid change
• Determination of Antihypertensive Peptides from an Izumi Shrimp Hydrolysate
• Transcriptional Regulation of Rat Calbindin Expression during Development Determined by Bacterially Expressed Protein
• IgE Responses in Mice Fed Moderate Protein Deficient and High Protein Diets
• Short-Chain Fatty Acids Increase the Level of Calbindin-D9k Messenger RNA in Caco-2 Cells

もっと見る 閉じる

スポンサーリンク