The Class V Chitin Synthase Gene csmA Is Crucial for the Growth of the chsA chsC Double Mutant in Aspergillus nidulans
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概要
- 論文の詳細を見る
chsA and chsC are genes encoding class II and I chitin synthases of Aspergillus nidulans respectively. In a previous study, chsA chsC double mutants showed various growth defects, suggesting that their cell wall architecture was disorganized and their cell wall integrity diminished. Here, we constructed chsA chsC chsD triple mutants and chsA chsC csmA triple mutants to investigate the role of the class IV and class V chitin synthases, ChsD and CsmA respectively, in maintaining the cell wall structure of the chsA chsC double mutant. The former triple mutant grew a little slower than the chsA chsC double mutant, but the two showed similar phenotypes. In contrast, the latter triple mutant exhibited severe growth defects, particularly under low osmotic conditions. The levels of the csmA transcript of the wild-type strain and chsA or chsC single mutants were markedly elevated under low osmotic conditions, while that of the chsA chsC double mutants was high even under such conditions. These and other results suggest that the function of csmA is important for the maintenance of cell wall integrity and the polarized growth of the chsA chsC double mutant.
- 社団法人 日本農芸化学会の論文
- 2005-01-23
著者
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Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Ohta A
Dep. Of Biotechnology The Univ. Of Tokyo
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Ohta A
Univ. Tokyo Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
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ICHINOMIYA Masayuki
Department of Biotechnology, The University of Tokyo
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Ogura A
Meiji University
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YAMADA Emi
Department of Biotechnology, The University of Tokyo
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Ichinomiya Masayuki
Department Of Biotechnology The University Of Tokyo
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Yamada Emi
Department Of Biotechnology The University Of Tokyo
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