Chicken Monoclonal Antibodies against Synthetic Bovine Prion Protein Peptide.

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概要

• 論文の詳細を見る

• Chicken monoclonal antibodies (mAbs) were developed against bovine prion protein (PrP) peptide. Chickens immunized with bovine PrP peptide B204 (amino acid residues 204-220) coupled to keyhole limpet hemocyanin produced specific antibodies to the peptide as determined by an enzyme-linked immunosorbent assay (ELISA) using the B204 peptide coupled to ovalbumin as target antigen. From a fusion experiment using the chicken fusion partner cell line MuH1 and immune spleen cells, 19 mAbs reactive with B204 were generated. These mAbs were subdivided into five groups based on competitive ELISA using B204 and four 10-amino acid peptides. These five groups included all combinations expected based on comparison of amino acid sequences among the five species, bovine, mouse, human, sheep and hamster, examined. These results indicate that the chicken mAb system is a suitable technique for immunological analysis of PrP in mammals.

• 公益社団法人 日本獣医学会の論文

著者

• FURUSAWA Shuichi

  Department of Immunobiology, Faculty of Applied Biological Science, Hiroshima University

• Furusawa Shuichi

  Department Of Immunobiology Faculty Of Applied Biological Science Hiroshima University

• Shinagawa Morikazu

  Department Of Hygiene And Microbiology Faculty Of Veterinary Medicine Hokkaido University

• Horiuchi Hiroyuki

  Department Of Agricultural Chemistry The University Of Tokyo

• Horiuchi Motohiro

  Department Of Epizootiology Faculty Of Veterinary Medicine Hokkaido University

• Matsuda Haruo

  Department Of Applied Chemistry Faculty Of Engineering Osaka University

• Matsuda Haruo

  Department of Immunobiology, Faculty of Applied Biological Science, Hiroshima University

• MATSUSHITA Kaori

  Department of Immunobiology, Faculty of Applied Biological Science, Hiroshima University

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