Cloning and Characterization of the Rhizopus niveus leul Gene and Its Use for Homologous Transformation
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概要
- 論文の詳細を見る
The Rhizopus niveus leul gene was cloned using a 2-kb HindIII fragment of the leuA gene of Mucor circinelloides as a hybridization probe. The coding region of this gene comprises 1890bp that encode a putative protein of 630 amino acids. The predicted amino acid sequence is similar to those of other α-isopropylmalate isomerases (α-IPMIs), showing 79.4%, 71.7%, and 60.6% identity with the Mucor circinelloides LeuA, Phycomyces blaskesleeanus Leu1, and Ustilago maydis Leu1, respectively. The vector pRN1, which contains a 3.7-kb BamHI-SacI fragment of the R. niveus leul gene, could complement leucine auxotrophy of R. niveus M-37 (leu1^-), suggesting that the gene codes for α-IPMI of R. niveus. Both the transformation frequency and mitotic stability with pRN1 were higher than those with leuA gene-containing vectors. It was found by using the leul Aene that the autonomously replicating sequence (ARS) of P. blakesleeanus partially functions as an ARS in R. niveus.
- 社団法人日本農芸化学会の論文
- 1996-03-23
著者
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Ohta Atsutane
Health & Bioscience Laboratories Meiji Seika Kaisha Ltd.
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Takagi M
Department Of Biotechnology The University Of Tokyo
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Ohta Akinori
Department of Agricultural Chemistry, The University of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Takaya N
Univ. Tsukuba Ibaraki Jpn
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Takaya Naoki
Department Of Biotechnology The University Of Tokyo:(present Address)institute Of Applied Biochemist
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Ohta A
Dep. Of Biotechnology The Univ. Of Tokyo
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Ohta A
Univ. Tokyo Tokyo
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Ohta Akinori
Department Of Agricultural Chemistry The University Of Tokyo
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HORIUCHI HIROYUKI
Department of Surgery, Kurume University School of Medicine
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Ogura Atsushi
Department Of Biotechnology The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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YANAI Koji
Department of Biotechnology, The University of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Horiuchi Hiroyuki
Department Of Biotechnology University Of Tokyo
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Horiuchi Hiroyuki
Earth Science Laboratory Faculty Of Education Hirosaki University
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Horiuchi Hiroyuki
Laboratory Of Cellular Genetics Department Of Biotechnology Division Of Agriculture And Agricultural
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Horiuchi Hiroyuki
Department Of Agricultural Chemistry The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Yanai Koji
Department Of Biotechnology The University Of Tokyo:bio Science Laboratories Meiji Seika Kaisha Ltd.
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Ogura A
Meiji University
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Takahi Masamichi
Department Of Biotechnology The University Of Tokyo
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YANAI Koji
Department of Agricultural Chemistry, The University of Tokyo
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