Isolation and Sequencing of a Gene, C-ADE1, and Its Use for a Host-Vector System in Candida maltosa with Two Genetic Markers(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
The host-vector systems of an n-alkane-assimiIating-yeast, Candida maltosa, that we previously constructed consisted of a vector replicating with an ARS region of this yeast, and C. maltosa strains J288 (leu2) or CH1 (his5) as hosts. Since each of these hosts has a single genetic marker, we have developed a new host-vector system using two genetic markers. By UV irradiation of strain CH1, an adenine auxotrophic mutant, CHA1, forming red colonies was isolated. A DNA fragment complementing this deficiency was isolated from the C. maltosa genome. Since the DNA fragment also complemented the ade1 mutation of S. cerevisiae, we termed a gene contained in this DNA fragment C-ADE1. The nucleotides of C-ADE1 were sequenced. The deduced amino acid sequence (291 residues) had 65.6% homology with that of ADE1 of S. cerevisiae (306 residues). Having the cloned C-ADE1 DNA, we improved the host-vector system of C. maltosa.
- 社団法人日本農芸化学会の論文
- 1991-01-23
著者
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Murao S
Department Of Agricultural Chemistry The University Of Tokyo
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Kawai Shinya
Department Of Orthopedic Surgery Yamaguchi University School Of Medicine
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YANO KEIJI
Department of BioEngineering, Nagaoka University of Technology
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Yano K
Dept. Bioeng. Nagaoka Univ. Tech.:jrdc
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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MURAO Satoru
Department of Agricultural Chemistry, The University of Tokyo
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HIKIJI Takeshi
Department of Agricultural Chemistry, The University of Tokyo
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Kawai Shinya
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Biotechnology The University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Takagi M
Department Of Biotechnology University Of Tokyo
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Murao Satoru
Department Of Agricultural Chemistry The University Of Tokyo
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Hikiji Takeshi
Department Of Agricultural Chemistry The University Of Tokyo
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