Evidence That More Than One Gene Encodes n-Alkane-inducible Cytochrome P-450s in Candida maltosa, Found by Two-Step Gene Disruption(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
An n-alkane-assimilating yeast, Candida maltosa, has a diploid genome. Probed with the previously isolated gene of n-alkane-inducible cytochrome P-450 (P-450alk), its allelic gene had been isolated, its nucleotides sequenced, and the interallelic divergence examined. Using one of the allelic genes, we disrupted the two alleles of the cytochrome P-450alk gene by a two-step gene disruption system. Surprisingly, the disruptant still assimilated n-alkane and contained n-alkane-inducible cytochrome P-450. This result indicates that, other than the disrupted two alleles, there is at least one other gene that encodes an n-alkane-inducible cytochrome P-450.
- 社団法人日本農芸化学会の論文
- 1991-07-23
著者
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Ohkuma Moriya
Department Of Agricultural Chemistry The University Of Tokyo
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Ohkuma Moriya
Bio-recycle Project Japan Science And Technology Agency:presto Japan Science And Technology Agency:e
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Ohkuma Moriya
Bio-recycle Project Japan Science And Technology Agency
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YANO KEIJI
Department of BioEngineering, Nagaoka University of Technology
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Tanimoto Takeshi
Department of Pediatrics National Cardiovascular Center
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Yano K
Dept. Bioeng. Nagaoka Univ. Tech.:jrdc
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Yano K
Univ. Tokyo Tokyo Jpn
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Takahashi Maki
Department Of Biotechnology The University Of Tokyo
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HIKIJI Takeshi
Department of Agricultural Chemistry, The University of Tokyo
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Takagi Masamichi
Department Of Biotechnology University Of Tokyo
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Tanimoto Takeshi
Department Of Agricultural Chemistry The University Of Tokyo
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Hikiji Takeshi
Department Of Agricultural Chemistry The University Of Tokyo
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SCHUNCK W.-H.
Central Institute of Molecular Biology
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MULLER H.-G.
Central Institute of Molecular Biology
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