Subcloniag and Nucleotide Sequencing of an ARS Site of Candida maltosa Which Also Functions in Saccharomyces cerevisiae
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概要
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A DNA region exhibiting ARS activity in Candida maltosa was subcloned from an isolated 3.8kb DNA fragment and designated as the TRA region previously [M. Takagi et al., J. Bacterial., 167, 551 (1986)], which replicates autonomously not only in C. maltosa but also in Saccharomyces cerevisiae. It was found that a DNA fragment of about 200bp (designated as fragment 2) exhibited ARS activity in both yeasts, and nucleotide sequence analysis of this fragment revealed that it contained five 11 bp-sequences which are homologous to the consensus sequence of ARS sites of S. cerevisiae [J. R. Broach et al., Cold Spring Harbor Symp. Quant. Biol., 47, 1167 (1982)] (9 among 11 bp being identical in each). Plasmids constructed using fragment 2 (pTRA2 and pTRA12) will be useful as cloning vectors, although they showed lower transforming frequencies and lower stabilities in C. maltosa than plasmids containing the TRA region (pTRA1 and pTRA11).
- 社団法人 日本農芸化学会の論文
著者
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Takagi Masamichi
Department of Agricultural Chemistry, The University of Tokyo
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Yano Keiji
Department Of Agricultural Chemistry Faculty Of Agriculture The University Of Tokyo
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Kawai Shinya
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied
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Takagi Masamichi
Department Of Agricultural Chemistry The University Of Tokyo
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Sugimoto Masakazu
Department Of Pharmacy Teikyo University Hospital
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HWANG Chel
Department of Agricultural Chemistry, The University of Tokyo
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