Detergent Alkaline Proteases : Enzymatic Properties, Genes, and Crystal Structures
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概要
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Subtilisin-like serine proteases from bacilli have been used in various industrial fields worldwide, particularly in the production of laundry and automatic dishwashing detergents. They belong to family A of the subtilase superfamily, which is composed of three clans, namely, true subtilisins, high-alkaline proteases, and intracellular proteases. We succeeded in the large-scale production of a high-alkaline protease (M-protease) from alkaliphilic Bacillus clausii KSM-K16, and the enzyme has been introduced into compact heavy-duty laundry detergents. We have also succeeded in the industrial-scale production of a new alkaline protease, KP-43, which was originally resistant to chemical oxidants and to surfactants, produced by alkaliphilic Bacillus sp. strain KSM-KP43 and have incorporated it into laundry detergents. KP-43 and related proteases form a new clan, oxidatively stable proteases, in subtilase family A. In this review, we describe the enzymatic properties, gene sequences, and crystal structures of M-protease, KP-43, and related enzymes
- 社団法人日本生物工学会の論文
- 2007-06-25
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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Saeki Katsuhisa
Tochigi Research Laboratories of Kao Corporation
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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Ito Susumu
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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Kawai S
Tochigi Research Laboratories Kao Corporation
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Kawai Shinya
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied
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Ito S
National Industrial Research Institute
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Kajita S
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Acriculture And Tech
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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