Isolation and Functional Analysis of the Promoter Sequence of the Cry j 1 Gene, Which Encodes a Major Allergenic Protein in the Pollen of Japanese Cedar (Cryptomeria japonica)
スポンサーリンク
概要
- 論文の詳細を見る
We isolated promoter sequences of the Cry j 1 gene-which encodes an allergenic protein in the pollen of Japanese cedar-by an inverse-PCR technique using genomic DNA as the template. One of the isolated promoter sequences conferred pollen-associated expression on a fusion construct that included a gene for β-glucuronidase (GUS) in transgenic tobacco plants. Histochemical analysis of GUS expression using four deletion mutants of this promoter sequence revealed that expression differed among pollen grains at different stages of maturation and that a 223-bp 5’-untranslated region of the gene was sufficient for spatial and temporal expression of the gene in pollen and pollen tubes. Our results indicate that the promoter sequence might be adequate for active pollen-associated expression in tobacco, as it is in Cryptomeria japonica.
- 日本植物細胞分子生物学会の論文
- 2003-09-01
著者
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KAJITA Shinya
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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MOROHOSHI Noriyuki
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Kawai S
Tochigi Research Laboratories Kao Corporation
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Kawai Shinya
Department Of Agricultural Chemistry The University Of Tokyo:(present Address) Department Of Applied
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Morohoshi Noriyuki
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Kajita Shinya
Graduate School Of Bio-application And Systems Engineering Tokyo University Of Agriculture & Tec
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YAMADA Shinya
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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SHIOKAWA Takahiro
Graduate School of Bio-Applications and Systems Engineering, Tokyo University of Agriculture and Tec
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Shiokawa Takahiro
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Yamada Shinya
Graduate School Of Bio-applications And Systems Engineering Tokyo University Of Agriculture And Tech
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Morohoshi Noriyuki
Graduate School of Bio-Application and Systems Engineering, Tokyo University of Agriculture & Technology
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