Purification and Characterization of Multiple Carboxymethyl Cellulases from Bacillus sp. KSM-522(Microbiology & Fermentation Industry)
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概要
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Three endo-β-1,4-glucanases (CMCases E-I, E-II and E-III) from Bacillus sp. KSM-522 were purified to homogeneity by repeated chromatography on DEAE Bio-Gel A. The molecular weights were 78,000 for E-I, and 61,000 for both E-II and E-III, as judged on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The optimum pH values for activity and isoelectric points were around 6 and 4.4 for E-I, and 7-10 and 3.5 for both E-II and E-III, respectively. These CMCases hydrolyzed CMC in a similar random fashion. The alkaline CMCases, E-II and E-III, were not distinguishable from each other with respect to temperature-activity curves, temperature-stability curves, substrate specificity, patterns of action on cellooligosaccharides, susceptibility to inhibitors or effects of metal ions.
- 社団法人日本農芸化学会の論文
- 1990-01-23
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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SHIKATA Shitsuw
Tochigi Research Laboratories of Kao Co.
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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OKOSHI Hiromi
Tochigi Research Laboratories of Kao Corporation
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OSHINO Kazushi
Tochigi Research Laboratories of Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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