Deduced Amino Acid Sequence and Possible Catalytic Residues of a Thermostable, Alkaline Cellulase from an Alkaliphilic Bacillus Strain
スポンサーリンク
概要
- 論文の詳細を見る
Alkaliphilic Bacillus sp. strain KSM-S237 (a relative of Bacillus pseudofirmus) produces a thermostable, alkaline endo-1,4-β-glucanase (Egl). The entire gene for the enzyme harbored a 2,472-bp open reading frame (ORF) encoding 824 amino acids, including a 30-amino-acid signal peptide. The deduced amino acid sequence of the mature enzyme (794 amino acids, 88,284 Da) showed very high similarity to those of family 5 mesophilic, alkaline Egls from some alkaliphilic bacilli. The enzyme had a region similar to a novel cellulose binding domain proposed for an Egl (EngF) from Clostridium cellulovorans. Expression of the Bacillus Egl gene in Bacillus subtilis resulted in high carboxymethy cellulase activity (2.0g/I) in the culture broth, concomitant with the appearance of a protein band on an SDS gel at 86 kDa. Site-directed mutagenesis delineated the importance of Arg^<111>, His^<151>, Glu^<190>, His^<262>, Tyr^<264>, and Glu^<305> in catalysis and/or substrate binding of the enzyme.
- 社団法人日本農芸化学会の論文
- 2000-11-23
著者
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Hatada Y
Japan Agency For Marine‐earth Sci. And Technol. (jamstec) Yokosuka Jpn
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Hatada Yuji
Tochigi Research Laboratories Of Kao Corporation
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Hayashi Y
Research Institute For Food Science Kyoto University
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Koike K
Faculty Of Pharmaceutical Sciences Toho University
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Koike Kenzo
Tochigi Research Laboratories, Kao Corporation
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Kobayashi Tohru
Extremobiosphere Research Center of Japan Agency for Marine-Earth Science and Technology (JAMSTEC)
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KOBAYASHI Tohru
Tochigi Research Laboratories of Kao Corporation
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HAKAMADA Yoshihiro
Tochigi Research Laboratories of Kao Corporation
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YOSHIMATSU Tadashi
Tochigi Research Laboratories of Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kobayashi T
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec)
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Koike K
Tochigi Research Laboratories Kao Corporation
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Ito S
National Industrial Research Institute
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Hayashi Y
Department Of Medicinal Chemistry Center For Frontier Research In Medicinal Science Kyoto Pharmaceut
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Kobayashi Tohru
Tochigi Research Laboratories Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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