Nucleotide Sequence of the Gene for an Alkaline Endoglucanase from an Alkalophilic Bacillus and Its Expression in Escherichia coli and Bacillus subtilis
スポンサーリンク
概要
- 論文の詳細を見る
The gene for an alkaline endoglucanase from the alkalophilic Bacillus sp. KSM-64 was cloned into the HindIII site of pBR322 and expressed in Escherichia coli HB101. The nucleotide sequence of a 4.1-kb region of the HindIII insert had two open reading frames, ORF-1 and ORF-2. The protein deduced from ORF-1 was composed of 244 amino acids with an M_r of 27,865. Subcloning analysis proved that the alkaline endoglucanase was encoded by ORF-2 (822 amino acids with an M_r of 91,040). Upstream from ORF-2,there were three consensus like sequences of the sigma A-type promoter of Bacillus subtilis, a putative Shine-Dalgarno sequence (AGGAGGT), and a catabolite repression operator-like seauence (TGTAAGCGGTTAACC). The HindIII insert was subcloned into a shuttle vector, pHY300PLK, and the encoded alkaline endoglucanase gene was highly expressed both in E.coli and B.subtilis. One of the three promoter-like sequences in ORF-2 could be suitable for high levels of enzyme expresson in both host organisms.
- 社団法人日本農芸化学会の論文
- 1992-06-23
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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SUMITOMO Nobuyuki
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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SUMITOMO Nobuyki
Tochigi Research Laboratories of Kao Corporation
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Sumitomo N
Tochigi Research Laboratories Of Kao Corporation
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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