HISTOCHEMICAL DISTRIBUTION OF GUANASE IN HUMAN TISSUES WITH GUANINE IN BICINE BUFFER AS SUBSTRATE
スポンサーリンク
概要
- 論文の詳細を見る
Histochemical studies of human guanase have seldom been undertaken, in part because of technical difficulties which result in heavy background staining. We previously reported a modified procedure in which the methodological inadequacies had been overcome. In this report, the modified technique has been applied to determine the distribution of guanase in normal human tissues and in cases of chronic hepatitis, hepatocellular carcinoma and metastatic tumors in liver (adenocarcinoma of colon). Guanase was present within the cytoplasm of hepatocytes throughout the entire lobule. Portal components (bile ducts and veins), fibrous tissue and inflammatory cells were non-reactive, and the enzyme was absent from hepatocyte nuclei in the normal liver. However, in the case of non A non B hepatitis nuclei presenting guanase activity or otherwise were identified. Guanase activity was observed for primary liver tumor but not for metastatic tumor. Moreover, guanase activity was observed in proximal tubuli of kidney and mucosal epithelium of small intestine. No activity was found in other tissues. The present method can be regarded as a useful method, not only to examine the specificity of guanase for hepatic diseases, but also to elucidate the physiological significance of guanase in the kidney and small intestine.
- 日本組織細胞化学会の論文
著者
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SANO Toshiaki
First Department of Pathology, the University of Tokushima
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II KUNIO
First Department of Pathology Tokushima University Hospital
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Ii Kunio
Second Department Of Internal Medicine Tokushima University
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Ii Kunio
First Department Of Pathology School Of Medicine The University Of Tokushima
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Sano Toshiaki
First Department Of Pathology School Of Medicine The University Of Tokushima
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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ITO SUSUMU
Second Department of Internal Medicine, School of Medicine, University of Tokushima
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Hayashi Kimiko
Second Department Of Internal Medicine The University Of Tokushima
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Tamura Yoshiyuki
Second Department of Internal Medicine, Tokushima University School of Medicine
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石原 昭彦
Laboratory Of Neurochemistry Graduate School Of Human And Environmental Studies Kyoto University
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Ishihara Akihiko
Second Department Of Internal Medicine The University Of Tokushima
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IWASAKI AKIHARU
Second Department of Internal Medicine, The University of Tokushima
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SYUNDO JYOJI
Second Department of Internal Medicine, The University of Tokushima
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Ii K
Second Department Of Internal Medicine Tokushima University
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Syundo Jyoji
Second Department Of Internal Medicine The University Of Tokushima
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Iwasaki Akiharu
Second Department Of Internal Medicine The University Of Tokushima
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Sano Toshiaki
First Department Of Internal Medicine And The First Department Of Pathology School Of Medicine Unive
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Tamura Yoshiyuki
Second Department Of Internal Medicine The University Of Tokushima
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Ito Susumu
Second Department Of Internal Medicine School Of Medicine University Of Tokushima
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Ishihara Akihiko
Laboratory Of Cell Biology And Life Science Graduate School Of Human And Environmental Studies Kyoto University
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Sano Toshiaki
First Department of Pathology School of Medicine, The University of Tokushima
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TAMURA Yoshiyuki
Second Department of Internal Medicine and the Division of Enzyme Regulation, Institute for Enzyme Research, Tokushima University
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