Hyperproduction of Sebaceous cis-6-Hexadecenoic Acid by Esterase-Reduced Mutant of Rhodococcus sp. Strain(MICROBIAL PHYSIOLOGY AND BIOTECHNOLOGY)
スポンサーリンク
概要
- 論文の詳細を見る
cis-6-Hexadecenoic acid is a major component of human sebaceous lipids that is involved in skin self-sterilization and atopic dermatitis amelioration. It can be prepared by hydrolysis of isopropyl cis-6-hexadecenoate produced by resting cells of Rhodococcus sp. strain KSM-MT66. To devise an economical industrial-scale process for the production of this rare fatty acid, we optimized the conditions for growing rhodococcal cells. Mg^<2+> and Fe^<2+> ions are essential for the efficient production of isopropyl cis-6-hexadecenoate. To further increase the production of isopropyl cis-6-hexadecenoate, we created a mutant strain (T64) with reduced esterase activity by random mutagenesis using UV irradiation of MT66. Under an optimized condition, the mutant T64 produced more than 60g l^<-1> isopropyl cis-6-hexadecenoate in a 4-d cultivation, corresponding to about 52 g l^<-1> cis-6-hexadecenoate.
- 社団法人日本生物工学会の論文
- 2007-10-25
著者
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ITO Susumu
Creative Research Initiative "Sousei" (CRIS), Hokkaido University
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Hagihara H
Tochigi Research Laboratories Kao Corporation
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Koike K
Faculty Of Pharmaceutical Sciences Toho University
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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Kobayashi Shunsuke
Department Of Electronic Engineering (div. Of Engineering Sciences) Faculty Of Technology Tokyo Univ
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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Araki Hiroyuki
Tochigi Research Laboratories, Kao Corporation
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Hagihara Hiroshi
Tochigi Research Laboratories, Kao Corporation
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Takigawa Hirofumi
Tochigi Research Laboratories, Kao Corporation
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Kotani Nobuharu
Tochigi Research Laboratories, Kao Corporation
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Tsujino Yukiharu
Tochigi Research Laboratories, Kao Corporation
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Koike Kenzo
Tochigi Research Laboratories, Kao Corporation
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Ozaki Katsuya
Tochigi Research Laboratories, Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Kobayashi Shunsuke
Science University Of Tokyo
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Koike K
Tochigi Research Laboratories Kao Corporation
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Ito S
National Industrial Research Institute
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Ozaki Katsuya
Tochigi Research Laboratories Of Kao Corporation
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Tsujino Y
Tochigi Research Laboratories Kao Corporation
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Araki Hiroyuki
Tochigi Research Laboratories Kao Corporation
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Araki H
Tochigi Research Laboratories Kao Corporation
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Kobayashi Shun-ichi
Department Of Physics School Of Science University Of Tokyo
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Ito Susumu
Creative Res. Initiative Sousei Hokkaido Univ.
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Kobayashi Shunichi
Department Of Physics Graduate School Of Science The University Of Tokyo
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Kotani Nobuharu
Tochigi Research Laboratories Kao Corporation
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Takigawa Hirofumi
Tochigi Research Laboratories Kao Corporation
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Hagihara Hiroshi
Tochigi Research Laboratories Kao Corporation
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KOBAYASHI Shun-ichi
Faculty of Engineering Science, Osaka University:(Present address)Department of Physics, University of Tokyo
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KOBAYASHI Shun-ichi
Department of Physics, University of Tokyo
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Kobayashi Shunichi
Department of Physics, Faculty of Science, University of Tokyo
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