Purification and Some Properties of an Alkaline Pullulanase from Alkalophilic Bacillus sp.KSM-1876
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概要
- 論文の詳細を見る
A novel alkaline pullulanase (pullulan 6-glucanohydrolase, EC3.2.1.41) was purified to homogeneity from the culture filtrate of the alkalophilic Bacillus sp.KSM-1876. The pullulanase had an optimum pH for activity of around 10.0-10.5,which is the highest pH for optimum activity of any pullulanases reported to date. The optimum temperature at pH 10 was around 50℃. The enzyme had a molecular mass of 120 kDa and an isoelectric point of pH 5.2. The enzyme acted specifically on pullulan to generate maltotriose as the major end product ; the K_m for pullulan was 1.8mg/ml. N-Bromosuccinimide abolished the enzymatic activity, and pullulan protected the enzyme from inactivation by this tryptophan-specific oxidant, suggesting that a tryptophan residue (s) is involved in the mechanism of action of the pullulanase from Bacillus sp.KSM-1876.
- 社団法人日本農芸化学会の論文
- 1992-01-23
著者
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Kawai S
Yamaguchi Univ. School Of Medicine Ube Jpn
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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KAWAI Shuji
Tochigi Research laboratories, Kao Corporation
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ITO Susumu
Tochigi Research laboratories, Kao Corporation
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Saeki Katsuhisa
Tochigi Research Laboratories of Kao Corporation
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IGARASHI Kazuaki
Tochigi Research Laboratories of Kao Corporation
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SAITO Kazuhiro
Tochigi Research Laboratories of Kao Corporation
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ARA Katsutoshi
Tochigi Research Laboratories of Kao Corporation
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Kawai Shuji
Tochigi Research Laboratories Kao Corporation
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Igarashi Kaori
Graduate School Of Human Life Sciences Showa Women's University
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Igarashi Kazuei
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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Kawai Shinya
Graduate School Of Engineering Tokyo University Of Agriculture And Technology
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Igarashi Kazuhiko
Graduate School Of Pharmaceutical Sciences Chiba University:amine Pharma Research Institute Innovati
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Ito Susumu
Tochigi Research Laboratories Kao Corporation
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