Construction of a Plasmid for High Level Expression of XMP Aminase in Escherichia coli(Microbiology & Fermentation Industry)
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概要
- 論文の詳細を見る
In order to produce 5'-guanylic acid (GMP) from 5'-xanthylic acid (XMP), we attempted to increase the activity of the conversion enzyme, XMP aminase (EC 6.3.4.1), in Escherichia coli. We subcloned the guaA gene coding for XMP aminase from plasmid pLC34-10 into pBR322. By connecting the tryptophan (trp) promoter at a suitable position upstream of the guaA gene, we obtained plasmid pXAR33. E. coli K294/pXAR33 showed an increase in activity of approximately 80 times when compared with K294, and the amount of the enzyme protein represented approx. 10% of the total cellular protein. The production of GMP with the plasmid carrying strain was also investigated.
- 社団法人日本農芸化学会の論文
- 1986-07-23
著者
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Ito S
Extremobiosphere Research Center Of Japan Agency For Marine-earth Science And Technology (jamstec):c
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NISHI Tatsunari
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Maruyama Akihiko
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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Maruyama Akihiko
Fine Chemicals Biochemicals Dept. Medicals Research And Development Center Kyowa Hakko Kogyo Co. Ltd
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Maruyama Akihiko
(present Address) Fermentation Research Institute Agency Of Industrial Science And Technology
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Ozaki Akio
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.:(present Office)technical Research Laboratori
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FUJIO Tatsuro
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Fujio T
Kyowa Hakko Kogyo Co. Ltd. Tokyo Jpn
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Fujio Tatsuro
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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ITO Seiga
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Nishi T
Hokkaido Univ. Sapporo Jpn
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Nishi Tatsunari
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd.
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