Enzymatic Formation of Plant Cerebroside : Properties of UDP-glucose : Ceramide Glu-cosyltransferase in Radish Seedlings
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概要
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The activity of cerebroside synthase (UDP-glucose : ceramide glucosyltransferase) was found in the microsomal fraction of radish hypocotyls, and was studied. One % of the radioactivity due to UDP-[3H]glucose added to the membrane fraction was incorporated into cerebroside within 60min. Optimum pH and temperature of the activity were pH 7. 8 and 30℃, respectively. No metal ions enhanced the cerebroside synthase activity, dissimilar to that in animal tissues. The apparent Km for UDP-glucose was approximately 200μM. The same activity was also observed in radish roots and cotyledons, but proportions of UDP-[3H]glucose incorporation were slightly lower than that in hypocotyls. Exogenous ceramide species having trihydroxy sphingoid bases, which were major ceramide components of radish cerebrosides, usually stimulated cerebroside formation in microsomal fractions from radish seedlings, while any ceramide types having dihydroxy sphingoid bases were ineffective on the glucosylation reaction. It was assumed, therefore, that cerebroside synthase in radish seedlings would have substrate selectivity for ceramide species.
- 社団法人日本農芸化学会の論文
- 1995-10-23
著者
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Ohnishi Masao
Department of Food Science, Obihiro University of Agriculture and Veterinary Medicine
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KOJIMA Michiyuki
Department of Animal Science, Obihiro University of Agriculture and Veterinary Medicine
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Ohnishi M
Obihiro Univ. Agriculture And Veterinary Medicine Obihiro Jpn
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Ohnishi M
Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici
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Kojima M
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Kojima Michiyuki
Department Of Animal Science Obihiro University Of Agriculture And Veterinary Medicine
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NAKAYAMA Masahiro
Department of Pathology, Osaka Medical Center and Research Institute for Maternal and Child Health
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Ohnishi M
Iwate Univ. Iwate Jpn
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Ohnishi Masao
Department Of Agricultural And Life Science Obihiro University Of Agriculture And Veterinary Medicin
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ITO Seisuke
Department of Bioresource Chemistry, Obihiro University of Agriculture and Veterinary Medicine
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Kojima M
Department Of Agricultural And Life Sciences Obihiro University Of Agriculture And Veterinary Medici
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Ito Seisuke
Department Of Bioresource Science Obihiro University Of Agriculture And Veterinary Medicine
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Sugimoto Isamu
Division Of Pathological Biochemistry Department Of Biomedical Sciences School Of Life Sciences Facu
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Nakayama Masahiro
Department Of Internal Medicine Division Of Cardiology Showa University Fujigaoka Hospital
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Nakayama M
Tokyo Univ. Sci. Noda‐shi Jpn
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Ohnishi M
Laboratory Of Applied Bioscience Obihiro University Of Agriculture And Veterinary Medicine
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Komai Michio
Nof Corporation
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Nakayama Masahiro
Department Of Applied Electronics Faculty Of Industrial Science And Technology Tokyo University Of S
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KOJIMA Michiyuki
Department of Agricultural Chemistry, Obihiro University of Agriculture and Veterinary Medicine
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ITO Seisuke
Department of Agricultural Chemistry, Obihiro University of Agriculture and Veterinary Medicine
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