Development of a Simple and Efficient Method for Transformation of Buckwheat Plants (Fagopyrum esculentum) Using Agrobacterium tumefaciens
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概要
- 論文の詳細を見る
Apical meristems of seedlings of buckwheat (Fagopyrum esculentum var. Shinano No.1) were pricked with a needle and inoculated with Agrobacterium tumefaciens (LBA4404,pBI121). The inoculated seedlings were grown to maturation and allowed to pollinate randomly to set the seeds (T1 plants). The transformation efficiency of the T1 plants was estimated by germination in the presence of geneticin (20 μg/ml) and by detection of β-glucuronidase (GUS) gene with PCR, indicating that 36% and 70% of the T1 plants were transformed, respectively. Four plants taking on a mutated morphology were selected from T1 plants which were transformed with the method using A. tumefaciens harboring a modified pBI121 for plasmid rescue. Southern blot analysis of plsasmids rescued from the 4 T1 plants demonstrated that each plasmid contained a different flanking DNA of the buckwheat genome, an evidence that T-DNA was integrated in different sites of the genomic DNA among the 4 T1 plants.
- 社団法人日本農芸化学会の論文
- 2000-04-23
著者
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Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Moriya Kei
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kojima Mineo
Department of Applied Biology, Shinshu University
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Kojima M
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Nozue Masayuki
Department of Biology, Faculty of Science, Shinshu University
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SHIOIRI Hidenari
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Kojima Michiyuki
Department Of Bioresource Chemistry Obihiro University Of Agriculture And Veterinary Medicine
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Kojima Mineo
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nozue Masayuki
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kojima M
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nogawa Masahiro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
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Shioiri Hidenari
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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ARAI Yukie
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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IWASE Narumi
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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SHIROTORI Kimiko
Department of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Arai Yukie
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Komai Michio
Nof Corporation
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Iwase Narumi
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Kojima Mineo
Department Of Applied Biological Science Faculty Of Textile Science And Technology Shinshu Universit
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Shirotori Kimiko
Department Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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