Expression of Two Trichoderma reesei Xylanases in the Fission Yeast Schizosaccharomyces pombe
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概要
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Two xylanase genes (xyn1 and xyn2) were amplified by the polymerase chain reaction (PCR) technique from first-strand cDNA prepared from mRNA of Trichoderma reesei QM9414. The genes were located under the human cytomegalovirus gene promoter (CMVp) on copy-number-controlled plasmids (pTLxyn1 and pTLxyn2). When both plasmids were introduced into Schizosaccharomyces pombe, functional xylanases (XYN I and XYN II) were secreted by the recombinant yeasts. The secreted XYN I protein had a molecular mass of 21 kDa whereas XYN II was produced as two molecular forms with sizes of 21 and 28 kDa, the former being not glycosylated and the latter N-glycosylated. XYN I was secreted in the culture medium at a level of about 25 μg/ml and XYN II at about 170 μg/ml. The recombinant xylanases had the same characteristics with respect to the effects of temperature and pH on the enzyme activity as the native ones.
- 社団法人日本生物工学会の論文
- 1999-11-25
著者
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Nogawa Masahiro
Division of Applied Biology, Faculty of Textile Science and Technology, Shinshu University
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Morikawa Yuko
Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T
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TAKANO Yoshihisa
Department of Respiratory Medicine, Tenri Hospital
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Okada Hidechika
Department Of Biodefense Medicine Nagoya City University Graduate School Of Medical Sciences
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Okada Hidechika
Department Of Biodefence Nagoya City University Graduate School Of Medical Science
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OKADA Hirofumi
Department of Bioengineering, Nagaoka University of Technology
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MORIKAWA Yasushi
Department of Bioengineering, Nagaoka University of Technology
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WAKAMATSU MEGUMI
Department of Bioengineering, Nagaoka University of Technology
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NOGAWA MASAHIRO
Department of Bioengineering, Nagaoka University of Technology
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Okada Hirofumi
Department Of Agricultural Chemistry College Of Agriculture University Of Osaka Prefecture
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Morikawa Yoshiyuki
Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T
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Nogawa Masahiro
Division Of Applied Biology Faculty Of Textile Science And Technology Shinshu University
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Nogawa Masahiro
Department Of Bioengineering Nagaoka University Of Technology
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Okada H
Choju Medical Institute Fukushimura Hospital
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Okada H
Department Of Biodefense Medicine Nagoya City University Graduate School Of Medical Sciences
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Morikawa Y
Department Of Bioengineering Nagaoka University Of Technology
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Morikawa Yasushi
Department Of Applied Physics And Information Science Mechanical Engineering Laboratory Agency Of In
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Takano Yoshihisa
Department Of Bioengineering Nagaoka University Of Technology
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Wakamatsu Megumi
Department Of Bioengineering Nagaoka University Of Technology
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