Purification and Characterization of Dipeptidyl Aminopeptidase from Aureobacterium sp. WO26
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概要
- 論文の詳細を見る
We isolated a bacterial strain with an enzyme which releases dipeptide from Gly-Arg-p-nitroanilide. The bacterium was tentatively identified as Aureobacterium sp. The enzyme, named AuDAP, was purified and characterized. It was homogenous by SDS-PAGE and IEF, and had a molecular mass of 90,000 Da by SDS-PAGE and 88,000 Da by gel filtration, so it may be a monomer. The isoelectric point was 3.8 and the optimum pH was 10.0. The purified enzyme hydrolyzed Gly-Arg-pNA, a model substrate for DAP I, and Arg-Arg-MNA, a model substrate for DAP III. However, this enzyme did not hydrolyze Gly-Phe pNA, also a model substrate for DAP I. These results suggested that this enzyme did not fall under the classification of mammalian DAPs and was similar to DAP BI from Pseudomonas sp. WO24 and dDAP from Dictyostelium discoideum, although several differences Were observed between them. The N-terminal amino acid sequence of this enzyme showed no significant homology to any enzyme and protein, except only for DAP BI.
- 社団法人日本農芸化学会の論文
- 1997-01-23
著者
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ANDO Katsuhiko
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd.
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Ochiai Keiko
Department of Pediatric Surgery Nagoya University Graduate School of Medicine
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Morikawa Yuko
Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T
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OGASAWARA WATARU
Department of Chemical Engineering, Faculty of Engineering, Iwate University
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Okada Hidechika
Department Of Biodefense Medicine Nagoya City University Graduate School Of Medical Sciences
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Okada Hidechika
Department Of Biodefence Nagoya City University Graduate School Of Medical Science
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Ando Katsuhiko
Tokyo Research Laboratories Kyowa Hakko Kogyo Co. Ltd
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MORIKAWA Yasushi
Department of Bioengineering, Nagaoka University of Technology
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Inanobe Noriyuki
Department of Bioengineering, Nagaoka University of Technology
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Okada Hirofumi
Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd
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Ochiai Keiko
Department Of Bioengineering Nagaoka University Of Technology
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Morikawa Yoshiyuki
Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T
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Okada H
Choju Medical Institute Fukushimura Hospital
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Okada H
Department Of Biodefense Medicine Nagoya City University Graduate School Of Medical Sciences
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Morikawa Y
Department Of Bioengineering Nagaoka University Of Technology
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Morikawa Yasushi
Department Of Applied Physics And Information Science Mechanical Engineering Laboratory Agency Of In
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Ogasawara W
Department Of Bioengineering Nagaoka University Of Technology
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Ogasawara Wataru
Department Of Applied Chemistry And Molecular Science Faculty Of Engineering Iwate University
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Inanobe Noriyuki
Department Of Bioengineering Nagaoka University Of Technology
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