Casein Kinase II (CK-II)-Mediated Stimulation of HIV-1 Reverse Transcriptase Activity and Characterization of Selective Inhibitors in Vitro
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概要
- 論文の詳細を見る
The physiological significance of the casein kinase II (CK-II)-mediated phosphorylation of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) on its three enzymatic activities [RNA-dependent DNA polymerase (RDDP), DNA-dependent DNA polymerase (DDDP) and ribonuclease H (RNase H)] was investigated in vitro. It was found that (i) the purified recombinant RT (rRT) functioned as an effective phosphate acceptor for CK-II; (ii) the RDDP, DDDP and RNase H activity of rRT was stimulated about 2.8-, 4.1- and 3.9-fold, respectively, after full phosphorylation by CK-II; and (iii) this stimulation was selectively inhibited by potent CK-II inhibitors, such as neocarzinostatin-chromophore (NCS-chrom) and three polyphenol-containing anti-oxidant compounds [quercetin, epigallocatechin gallate (EGCG) and 8-chloro-3', 4', 5,7-tetrahydroxyisoflavone (8C-3', 4', 5,7-THI)]. These results suggest that (i) CK-II may be responsible for activation of RT in HIV-1-infected cells; and (ii) the selective inhibition of CK-II-mediated activation of HIV-1 RT by potent CK-II inhibitors may be involved in the mechanism of their anti-HIV-1 effects at the cellular level.
- 公益社団法人日本薬学会の論文
- 1999-10-15
著者
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MAEKAWA Toshiro
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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OHTSUKI Kenzo
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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原田 俊司
Meiji College of Pharmacy
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NAGATA NOBUYUKI
Research Laboratory, Minophagen Co.
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Nagata Nobuyuki
Research Laboratories Of Minophagen Pharmaceutical Co. Ltd.
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Morikawa Yuko
Section Of Virus The Kitasato Institute
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Morikawa Yuko
Laboratory Of Food Science And Nutrition Department Of Life Style Studies School Of Human Cultures T
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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FUNAYAMA Shinji
Department of Kampo Pharmaceutical Sciences, Nihon Pharmaceutical University
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HARADA Shigeyoshi
Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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HANEDA Eiji
Laboratory of Genetical Biochemistry, School of Allied Health Sciences, Kitasato University
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Haneda Eiji
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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永田 尚也
Gifu Pharmaceutical University
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Funayama S
Dep. Of Kampo Pharmaceutical Sciences Nihon Pharmaceutical Univ.
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Funayama Shinji
Department Of Bioscience And Biotechnology
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Maekawa Toshiro
Laboratory For Digestive Diseases Center For Genomic Medicine Riken
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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