Characterization of Human Lactoferricin as a Potent Protein Kinase CK2 Activator Regulated by A-Kinase in Vitro
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概要
- 論文の詳細を見る
Lactoferricin(LFcin)hydrolyzed from lactoferrin(LF), a major 80 kDa iron-binding protein in milk and other exocrine secretions, was characterized as a potent activator of protein kinase CK2(CK2)in vitro. Human LFcin(hLFcin)at 0.5μg stimulated approx. 5-fold CK2 activity[phosphorylation of 60S acidic ribosomal proteins(P0, P1, P2)and Hsp90(p98)]in a manner similar to other functional proteins with oligo-Arg clusters, such as salmine A1, sperm histone H2B and HIV-1 Rev. Interestingly, this stimulatory effect of hLFcin was significantly reduced when it was phosphorylated by A-kinase in vitro. These results suggest that(i)hLFcin acts as a potent CK2 activator in vitro; and(ii)the stimulatory effect of hLFcin on CK2 activity is regulated by its phosphorylation by A-kinase in vitro.
- 2002-01-01
著者
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MAEKAWA Toshiro
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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FUJIHARA Michio
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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OHTSUKI Kenzo
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Fujihara Michio
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry Graduate School Of Medical Sciences Kitasato University
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Maekawa Toshiro
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Maekawa Toshiro
Laboratory For Digestive Diseases Center For Genomic Medicine Riken
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Otsuki Kenzo
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasato University
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