Characterization of Bovine and Human Lactoferrins as Glycyrrhizin-Binding Proteins and Their Phosphorylation in Vitro by Casein Kinase II
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概要
- 論文の詳細を見る
The binding ability of bovine and human lactoferrins (bLF and hLF; LFs) to a glycyrrhizin (GL)-affinity column and their phosphorylation by casein kinase II (CK-II) in vitro were biochemically investigated. It was found that (i) both bLF and hLF are GL-binding proteins; (ii) purified both proteins function as phosphate acceptors of CK-II; and (iii) this phosphorylation is completely inhibited by two polyphenol-containing anti-oxidant compounds (quercetin and epigallocatechin gallate) at 1 μM, whereas a glycyrrhetinic acid derivative (oGA) inhibits it at one tenth the concentration of GL. The DNA-binding affinity of hLF was reduced by GL in a dose dependent manner. However, no significant effect of the CK-II-mediated hLF phosphorylation on its DNA-binding affinity was detected. These results suggest that the GL-induced inhibition of the DNA-binding affinity and the CK-II-mediated phosphorylation of hLF may be closely correlated with the anti-inflammatory effect of GL in the human body.
- 公益社団法人日本薬学会の論文
- 2000-10-01
著者
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FUJIHARA Michio
Laboratory of Genetical Biochemistry and Signal Biology, Graduate School of Medical Sciences, Kitasa
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Tanaka Katsunao
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Tanaka Katsunao
大鵬薬品工業
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Tanigawa Kiyoshi
大鵬薬品工業
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Tanigawa Kiyoshi
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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Ohtsuki K
Kitasato Univ. Sagamihara Jpn
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Ohtsuki Kenzo
Genetical Biochem. Kitasato Univ.
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ITO Junko
Laboratory of Biochemistry, Sagami Women's University
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Fujihara M
Kitasato Univ. Sagamihara Jpn
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Fujihara Michio
Laboratory Of Genetical Biochemistry School Of Allied Health Sciences Kitasato University
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Fujihara Michio
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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大久保 研之
Department Of Clinical Chemistry And Laboratory Medicine Faculty Of Medicine Kyushu University
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Ohtsuki K
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Tanigawa Kozo
Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.
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YANAHIRA Shuichi
Technology and Research Institute, Snow Brand Milk Products Co., Ltd.
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HATOMI Masahito
Laboratory of Genetical Biochemistry Kitasato University
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TANIGAWA Kozo
Clinical Chemistry Kitasato University
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OHTSUKI Kenzo
Clinical Chemistry Kitasato University
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Shimoyama Y
Kitasato Univ. Sagamihara Jpn
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Yanahira Shuichi
Technology And Research Institute Snow Brand Milk Products Co. Ltd.
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Ohtsuki Kenzo
Laboratory Of Genetical Biochemistry And Signal Biology Graduate School Of Medical Sciences Kitasato
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Ito Junko
Laboratory Of Biochemistry Sagami Women's University Kitasato University
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YANAHIRA Shuichi
Technical Research Institute, Snow Brand Milk Products Co. Ltd.
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