Construction of the Single-Chain Fv from 196-14 Antibody toward Ovarian Cancer-Associated Antigen CA125

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概要

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• The variable regions of heavy- and light-chains of mouse monoclonal antibody 196-14 toward ovarian cancer-associated antigen CA125 were linked with a peptide linker (GSTSGSGKSSEGKG) and a histidine tag was attached at the carboxyl terminal. This single-chain Fv (scFv) with a histidine tag was expressed in Escherichia coli as an inclusion body. The inclusion body was solubilized with guanidium chloride, followed by purification on nickel nitrilotriacetic acid agarose column and refolding into the active form. The scFv thus obtained bound to the Siso cells, which express CA125,and may recognize the same epitope as the parental 196-14 antibody IgG does.

• 社団法人日本薬学会の論文
• 1999-10-15

著者

• Nishimura M

  First Department Of Medicine Hokkaido University School Of Medicine

• NAKASHIMA Manabu

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• WATANABE Takeshi

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• Tanaka Katsunao

  Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.

• Tanaka Katsunao

  大鵬薬品工業

• Tanigawa Kiyoshi

  大鵬薬品工業

• Tanigawa Kiyoshi

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Tanigawa Kiyoshi

  Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.

• Ueda T

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• Ueda T

  Graduate School Of Pharmaceutical Sciences Kyushu University

• IMOTO Taiji

  Faculty of Biotechnology and Life Science, Sojo University

• UEDA Tadashi

  Graduate School of Pharmaceutical Sciences, Kyushu University

• IMOTO Taiji

  Graduate School of Pharmaceutical Sciences, Kyushu University

• HASHIMOTO Yoshio

  Graduate School of Pharmaceutical Sciences, Kyushu University

• SONODA Kenzo

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• Nakashima Manabu

  Department Of Molecular Immunology Medical Institute Of Bioregulation Kyushu University

• Imoto T

  Faculty Of Biotechnology And Life Science Sojo University

• Imoto Taiji

  Graduate School Of Pharmaceutical Sciences

• Imoto Taiji

  Graduate School Of Pharmaceutical Science Kyushu University

• Sonoda Kenzo

  Department Of Molecular Immunology Medical Institute Of Bioregulation Kyushu University

• Ueda Takuya

  Department Of Chemistry And Biotechnology Graduate School Of Engineering The University Of Tokyo

• 上田 卓也

  東京大学大学院新領域創成科学研究科メディカルゲノム専攻

• Ueda Tadashi

  Graduate School Of Pharmaceutical Sciences Kyushu Univ.

• Ueda Tadashi

  Graduate School Of Pharmaceutical Sciences

• Imoto Taiji

  九州大学 薬学研究院

• Hashimoto Y

  Graduate School Of Pharmaceutical Sciences Kyushu University

• Nakashima M

  Kyushu Univ. Fukuoka Jpn

• Hashimoto Yuichi

  Institute Of Molecular & Cellular Biosciences University Of Tokyo

• Tanigawa Kozo

  Pharmacobioregulation Research Laboratory Taiho Pharmaceutical Co. Ltd.

• Nakashima Manabu

  Department Of Gastroenterology National Hospital Organization Kyushu Medical Center

• Watanabe Takeshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Watanabe Takeshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• Hashimoto Yoshio

  Graduate School Of Pharmaceutical Sciences Kyushu University

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