Comparative Studies of β-1,3-Glucanase A1 and B of Bacillus circulans WL-12 : Purifications and Enzymatic Properities

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概要

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• The β-1,3-glucan-degrading enzymes of Bacillus circulans WL-12 induced with pachyman, a β-1,3-glucan, consist of β-1,3-glucanase A1 (GlcA1), β-1,3-glucanase B(GlcB) and derivatives of these two glucanases. In order to compare enzymatic properties of the two glucanases, GlcA1 produced by Escherichia coli HB101 carrying a cloned glcA gene, and GlcB produced by both B. circulans WL-12 and E. coli HB101 carrying a cloned glcB gene were purified. Enzymatic properties of purified GlcA1 and GlcB were compared and striking differences were found in the length distributions of the hydrolysis products and the substrate specificities. Purified GlcA1 hydrolyzed pachyman and generated laminarioligosaccharides, from G1 to G5. On the other hand, pachyman hydrolysis by GlcB generated shorter laminarioligosaccharides, G1,G2 and G3. GlcB exhibited higher hydrolyzing activity against mixed-linkage β-1,3-1,4-glucans than that against β-1,3-glucan, while GlcA1 did not show any activity on mixed-linkage β-1,3-1,4-glucans.

• 社団法人日本生物工学会の論文
• 1995-09-25

著者

• WATANABE Takeshi

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• Okada Takeshi

  Deparment of Cardiology, Osaka General Medical Center

• Tanaka H

  Department Of Bioresources Chemistry Faculty Of Agriculture Okayama University

• NIKAIDOU Naoki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Watanabe T

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• TANAKA Hirosato

  Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University

• Okada T

  Tsukuba Univ. Ibaraki Jpn

• AIDA KAZUHIKO

  Department of Applied Biological Chemistry, Faculty of Agiculture, Niigata University

• KASAHARA NOBUO

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Nikaidou N

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Nikaidou Naoki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Tanaka Hirosato

  Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University

• Aida Kazuhiko

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Kasahara Nobuo

  Department Of Pharmacology Taiho Pharmaceutical Co. Ltd.

• Watanabe Takeshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Watanabe Takeshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

• AIDA Kazuhiko

  Department of Agricultural and Life Science, Obihiro University of Agriculture and Veterinary Medicine

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