Sensitization of the Adenylyl Cyclase System in Cloned κ-Opioid Receptor-Transfected Cells Following Sustained Agonist Treatment : A Chimeric Study Using G Protein α_<i2>/α_q Subunits
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概要
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Chronic and/or sustained opioid treatment has been shown to result in development of sensitization of the adenylyl cyclase (AC) system or cAMP overshoot. In this study, we investigated the molecular mechanism responsible for sensitization of the AC system using CHO cells co-expressing cloned κ-opioid receptor and some chimeric G protein αi2/αq subunits. In CHO cells co-expressing the κ-opioid receptor and pertussis toxin-insensitive chimeric αi2/αq subunits with αi2 residues Met244 - Asn331, despite pretreatment with pertussis toxin, acute treatment with the κ-opioid-receptor - selective agonist U69, 593 suppressed forskolin-stimulated cAMP accumulation, while sustained treatment with U69, 593 (4 h) induced cAMP overshoot over the naive level by the κ-opioid-receptor - selective antagonist norbinaltorphimine (sensitization of the AC system). On the other hand, in CHO cells co-expressing the κ-opioid receptor and pertussis toxin-insensitive chimeric αi2/αq subunits without αi2 residues Met244 - Asn331, pretreatment with pertussis toxin completely blocked these acute and sustained effects of U69, 593 on cAMP accumulation. These results suggested that the presence of the specific region of αi2 (Met244 - Asn331), which was reported to be responsible for the inhibition of AC, and continuous inhibition of AC by αi2 is necessary for the development of sensitization.
- 社団法人 日本薬理学会の論文
- 1999-12-01
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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NAKAGAWA Takayuki
Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University
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SATOH Masamichi
Department of molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto university
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Nakagawa Takayuki
Department Of Molecular Pharmacology Graduate School Of Pharmaceutical Sciences Kyoto University
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Nakagawa Takayuki
Department Of Fisheries Faculty Of Agriculture Kinki University
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MINAMI Masabumi
Department of Molecular Pharmacology, Graduate School of Pharmaceutical Sciences, Kyoto University
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Minami Masabumi
Department Of Molecular Pharmacology Faculty Of Pharmaceutical Sciences Kyoto University
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Nakagawa Takayuki
Dep. Of Molecular Pharmacology Graduate School Of Pharmaceutical Sciences Kyoto Univ. Jpn
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OZAWA Tohru
Department of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Kyoto University
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Ozawa T
Department Of Molecular Pharmacology Faculty Of Pharmaceutical Sciences Kyoto University
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Ozawa Tohru
Department Of Applied Physics Waseda University
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Satoh Masamichi
Department Of Molecular Pharmacology Faculty Of Pharmaceutical Science Kyoto University
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Satoh Masamichi
Yasuda Women's Univ. Jpn
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Watanabe T
Tokyo Univ. Fisheries Tokyo Jpn
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Sato Masamichi
Department Of Hygiene And Preventive Medicine Faculty Of Medicine Saitama Medical School
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Satoh Masamichi
Department Of Hygiene And Preventive Medicine Faculty Of Medicine Saitama Medical School
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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