Site-directed Mutagenesis of the Asp-197 and Asp-202 Residues in Chitinase A1 of Bacillus circulans WL-12
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概要
- 論文の詳細を見る
The contribution of the Asp-197 and Asp-202 residues in chitinase A1 of Bacillus circulans WL-12 to the catalytic reaction was studied by site-directed mutagenesis of these residues. A kinetic analysis of the purified mutant chitinases suggests the involvement of both the Asp-197 and Asp-202 residues in the catalytic events of this enzyme, although the effects of mutations of Asp-197 were less severe than those of the other mutations.
- 社団法人日本農芸化学会の論文
- 1994-12-23
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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UCHIDA Makoto
Department of Biology, Faculty of Science, Chiba University
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Watanabe T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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TANAKA Hirosato
Department of Agricultural Chemistry, Faculty of Agriculture, Niigata University
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Uchida M
Department Of Food Science And Nutrition School Of Human Life And Environmental Sciences Mukogawa Wo
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Tanaka H
National Inst. Agrobiological Sci. Ibaraki Jpn
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Tanaka Hirosato
Department Of Agricultural Chemistry Faculty Of Agriculture Niigata University
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Kobori Kiichiro
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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Kobori Kiichiro
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Uchida Makoto
Department Of Biology Faculty Of Science Chiba University
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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Uchida Makoto
Department Of Applied Biological Chemistry College Of Agriculture Osaka Prefecture Unviersity
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