Purification and Characterization of a Rice Class I Chitinase, OsChia1b, Produced in Esherichia coli
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概要
- 論文の詳細を見る
To determine the properties and structure of OsChia1b, a family 19 chitinase from Oryza sativa L. cv. Nipponbare (japonica ssp.), recombinant OsChia1b was produced in Esherichia coli cells and purified to homogeneity by chitin affinity column chromatography. OsChia1b was highly active against soluble chitinous substrate, but not against crystalline chitin, and clearly inhibited hyphal extension of Trichoderma reesei.
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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Nishizawa Yoko
Division of Plant Sciences, National Institute of Agrobiological Sciences
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Mizuno Ryoji
Department of Surgery, The Jikei University School of Medicine
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Suzuki Kazushi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University:(present Addres
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Nonaka Takamasa
School Of Pharmacy Iwate Medical University
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ITOH Yoshikane
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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KEZUKA Yuichiro
School of Pharmacy, Iwate Medical University
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