Construction of a Binary Vector for Knockout and Expression Analysis of Rice Blast Fungus Genes
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概要
- 論文の詳細を見る
We developed an efficient method to analyze gene function and expression of the rice blast fungus. We constructed a GATEWAY binary vector, which generates a gene-targeted disruptant carrying a green fluorescent protein gene under the native promoter of the target gene. Using this method, the knockout efficiency and expression patterns of two hypothetical genes were determined.
- 社団法人 日本農芸化学会の論文
著者
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KUBO Yasuyuki
Laboratory of Plant Pathology, Graduate School of Agriculture, Kyoto Prefectural University
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Hayashi Nagao
Division of Plant Sciences, National Institute of Agrobiological Sciences
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Nishizawa Yoko
Division of Plant Sciences, National Institute of Agrobiological Sciences
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MINAMI Eiichi
Division of Plant Sciences, National Institute of Agrobiological Sciences
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SAITOH Ken-ichiro
Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)
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NISHIMURA Marie
Division of Plant Sciences, National Institute of Agrobiological Sciences (NIAS)
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