Comparison of Enzymatic and Antifungal Properties between Family 18 and 19 Chitinases from S. coelicolor A3(2)

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概要

• 論文の詳細を見る

• Streptomyces coelicolor A3(2) has 13 chitinase genes encoding 11 family 18 and two family 19 chitinases. To compare enzymatic properties of family 19 chitinase and family 18 chitinases produced by the same organism, the four chitinases (Chi18bA, Chi18aC, Chi18aD, and Chi19F), whose genes are expressed at high levels in the presence of chitin, were produced in Eschericha coli and purified. The effect of pH on the hydrolytic activity was very different not only among the four chitinases but also among the substrates. The hydrolytic activity of Chi19F, family 19 chitinase, against soluble substrates was remarkably high as compared with three family 18 chitinases, but was the lowest against crystalline substrates among the four chitinases. On the contrary, Chi18aC, a family 18-subfamily A chitinase, showed highest activity against crystalline substrates. Only Chi19F exhibited significant antifungal activity. Based on these observations, the roles of family 19 chitinases are discussed.

• 社団法人 日本農芸化学会の論文
• 2006-04-23

著者

• WATANABE Takeshi

  Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University

• Fujii T

  Kyoto Univ. Kyoto

• Fujii T

  Department Of Applied Biological Chemistry The University Of Tokyo

• Saito Akiko

  Biotechnology Center Toyama Prefecture

• Saito Akiko

  Department Of Applied Biological Chemistry The University Of Tokyo

• Fujii Takeshi

  National Institute for Agro-Environmental Sciences

• Fujii T

  Inst. Biol. Sci. Univ. Tsukuba

• NIKAIDOU Naoki

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• Watanabe T

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• KAWASE Tomokazu

  Department of Biosystem Science, Graduate School of Science and Technology, Niigata University

• Fujii T

  Div. Of Bioresource Sci. Graduate School Of Horticulture Chiba Univ.

• Nikaidou N

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Nikaidou Naoki

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Kawase Tomokazu

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• YOKOKAWA Shinya

  Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University

• SAITO Akihiro

  National Institute for Agro-Environmental Sciences

• MIYASHITA Kiyotaka

  National Institute for Agro-Environmental Sciences

• Yokokawa Shinya

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Miyashita K

  National Institute For Agro-environmental Sciences

• Miyashita Kiyotaka

  National Inst. For Agro-environmental Sciences

• Watanabe Takeshi

  Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University

• Fujii Takeshi

  National Inst. For Agro-environmental Sciences

• Watanabe Takeshi

  Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University

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