Site-Directed Mutagenesis of Asp280 Suggests Substrate-Assisted Catalysis of Chitinase A1 from Bacillus circulans WL-12
スポンサーリンク
概要
- 論文の詳細を見る
To study the involvement of Asp280 of chitinase A1 from Bacillus circulans WL-12 in the catalytic reaction of the enzyme, site-directed mutagenesis of this residue was carried out. Kinetic analysis of the mutant chitinases revealed that the residue is not absolutely essential for the catalytic reaction and thus, does not act as the catalytic nucleophile.
- 社団法人日本生物工学会の論文
- 2000-01-25
著者
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WATANABE Takeshi
Department of Molecular Immunology, Medical Institute of Bioregulation, Kyushu University
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HASHIMOTO Masayuki
Department of Mechanical Systems Engineering, Tokyo University of Agriculture and Technology
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NIKAIDOU Naoki
Department of Applied Biological Chemistry, Faculty of Agriculture, Niigata University
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FUKAMIZO Tamo
Laboratory of Enzyme System Science, Faculty of Agriculture, Kinki University
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Hashimoto Masayuki
Department Of Applied Science For Electronics And Materials Graduate School Of Engineering Sciences
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Watanabe T
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Fukamizo T
新潟大学 農学部応用生物化学科
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Fukui T
Faculty Of Agriculture Kinki University
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Fukamizo Tamo
Laboratory Of Biophysical Chemistry Faculty Of Agriculture Kinki University
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Nikaidou Naoki
Department Of Biosystem Science Graduate School Of Science And Technology Niigata University:departm
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Nikaidou Naoki
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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HONDA Yuji
Laboratory of Bioorganic Chemistry, College of Agriculture, Osaka Prefecture University
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Hashimoto M
Kddi R&d Laboratories Inc.
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Watanabe Takeshi
Department Of Applied Biological Chemistry Faculty Of Agriculture Niigata University
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Honda Yuji
Laboratory Of Bioorganic Chemistry Faculty Of Agriculture Osaka Prefecture University
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Honda Yuji
Laboratory Of Bioorganic Chemistry College Of Agriculture Osaka Prefecture University
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Watanabe Takeshi
Department Of Agricultural Chemistry Faculty Of Agriculture Tohoku University
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